| 货号 | BAF2419 |
| 别名 | Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1 | 全称 | Pancreas/Duodenum Homeobox-1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human PDX‑1/IPF1 in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3651 (Human); 18609 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PDX‑1/IPF1 Ala91-Arg283 Accession # P52945 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is ahomeodomain-containing transcription factor. During embryonicdevelopment, PDX-1 is required for pancreas differentiation in adultislet cells. PDX-1 regulates beta -cell specific gene expression andfunction. Human and mouse PDX-1 share 88% amino acid sequence homology. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
