| 货号 | BAF225 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB625-500；Detection antibody：BAF225; and protein: 636-R1-025 |
| 别名 | CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor | 全称 | Tumor Necrosis Factor Receptor I |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects human TNF RI in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant human (rh) TNF‑ alpha, recombinant mouse (rm) TNF‑ alpha, recombinant porcine TNF‑ alpha, recombinant rat TNF‑ alpha, rhTNF‑ beta, rmsTNF RI, rhsTNF RII, and rmsTNF RII is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7132 (Human); 21937 (Mouse); 25625 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Aminopeptidase P3, a new member of the TNF-TNFR2 signaling complex, induces phosphorylation of JNK1 and JNK2. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human sTNF RI |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily member, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF- alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1 - 3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6 - 8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa ECD with a PLAD domain(5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of human TNF RI shows 80%, 80%, 73%, 69% and 70% aa identity with dog, cat, pig, rat and mouse TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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