| 货号 | BAF223 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB623；Detection antibody：BAF223; and protein: 223-2A-005 |
| 别名 | CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR | 全称 | Interleukin 2 Receptor alpha |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) IL‑2, rmIL‑2, rpIL‑2, rrIL‑2, rhIL‑2 R beta, and rhIL‑2 R gamma is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | recombinant human CD25/IL-2 R alpha extracellular domain |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human IL-2 receptor alpha (IL-2 R alpha ), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|