Human TNF-alpha Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human TNF-alpha Biotinylated Affinity Purified PAb (50 UG)

货号： BAF210 基本售价： 5532.3 元规格： -

产品信息

概述
货号	BAF210
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB610 or MAB610R-100；Detection antibody：BAF210; and protein: 210-TA-010
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Human
应用	Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair)
目标/特异性	Detects human TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) TNF‑ beta, recombinant mouse (rm) TNF-alpha, recombinant rat TNF‑ alpha, recombinant porcine TNF-alpha, rhsTNF RII, rmsTNF RI, and rmsTNF RII is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcgammaR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment. Authors: Matt P, Lindqvist U, Kleinau S PLoS ONE, 2015;10(9):e0137474. Species: Human Sample Type: Cell Culture Supernates Application: ELISA detection C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY Diabetologia, 2011;54(10):2713-23. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Protection from RNA and DNA viruses by IL-32. Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF J. Immunol., 2011;186(7):4110-8. Species: Human Sample Type: Cell Culture Supernates Application: Electrochemiluminescence Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Development and validation of sandwich ELISA microarrays with minimal assay interference. Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA J. Proteome Res., 2008;7(6):2406-14. Species: Human Sample Type: Serum Application: ELISA Microarray Development A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects. Authors: OToole M, Legault H, Ramsey R, Wynn TA, Kasaian MT Clin. Exp. Allergy, 2008;38(4):594-601. Species: Human Sample Type: BALF Application: ELISA Development Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. Authors: Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK J. Immunol., 2008;180(4):2553-61. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Clin. Chem., 2007;53(11):2010-2. Species: Human Sample Type: Plasma Application: ELISA Development Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Ultrasensitive flow-based immunoassays using single-molecule counting. Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Clin. Chem., 2007;53(11):1990-5. Species: Human Sample Type: Plasma Application: ELISA Development HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P AIDS, 2007;21(10):1263-72. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Increased ENA-78 in the follicular fluid of patients with endometriosis. Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA Acta Obstet Gynecol Scand, 2006;85(3):336-42. Species: Human Sample Type: Follicular Fluid Application: ELISA Development Selective regulation of IL-10 signaling and function by zymosan. Authors: Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB J. Immunol., 2006;176(8):4785-92. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Sepsis and pathophysiology of anthrax in a nonhuman primate model. Authors: Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S Am. J. Pathol., 2006;169(2):433-44. Species: Primate â€“ Papio cynocephalus (Baboon) Sample Type: Plasma Application: ELISA Development trans fatty acids and systemic inflammation in heart failure. Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC Am. J. Clin. Nutr., 2004;80(6):1521-5. Species: Human Sample Type: Plasma Application: ELISA Development IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis. Authors: Sharif MN, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv LB J. Immunol., 2004;172(10):6476-81. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. Authors: Copeland S, Siddiqui J, Remick D J. Immunol. Methods, 2004;284(1):99-106. Species: Human Sample Type: Cell Culture Supernates Application: Array Development Reprogramming of IL-10 activity and signaling by IFN-gamma. Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB J. Immunol., 2003;171(10):5034-41. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human TNF-alpha (R&D Systems, Catalog # 210-TA) Val77-Leu233 Accession # P01375
生物活性	Human
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Human TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. Pennica, D. et al. (1984) Nature 312:724. Tang, P. et al. (1996) Biochemistry 35:8216. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. Black, R.A. et al. (1997) Nature 385:729. Moss, M.L. et al. (1997) Nature 385:733. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335. Romanatto, T. et al. (2007) Peptides 28:1050. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

参考图片

TNF‑ alpha in Human PBMCs. TNF‑ alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF‑ alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF210) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.