Human IL-6 Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

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Human IL-6 Biotinylated Affinity Purified PAb (50 UG)

货号： BAF206 基本售价： 5532.3 元规格： -

产品信息

概述
货号	BAF206
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB206；Detection antibody：BAF206; and protein: 206-IL-010
别名	B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta 2; IFN-beta-2; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
全称	Interleukin 6
反应种属	Human
应用	Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard
目标/特异性	Detects human and primate IL-6 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant porcine IL‑6 is observed and less than 0.01% cross-reactivity with recombinant mouse IL-6 and recombinant rat IL-6 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The Human Antimicrobial Protein Bactericidal/Permeability-Increasing Protein (BPI) Inhibits the Infectivity of Influenza A Virus Authors: Olaf Pinkenburg PLoS ONE, 2016;11(6):e0156929. Species: Human Sample Type: Cell Culture Supernates Application: ELISA detection Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition. Authors: Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J Endocrinology, 2015;156(2):670-9. Species: Human Sample Type: Cell Culture Supernates Application: ELISA detection Protection from RNA and DNA viruses by IL-32. Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF J. Immunol., 2011;186(7):4110-8. Species: Human Sample Type: Cell Culture Supernates Application: Electrochemiluminescence Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines. Authors: Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE AIDS Res. Hum. Retroviruses, 2008;24(5):667-71. Species: Human Sample Type: Vaginal Fluid Application: ELISA Development Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma. Authors: Osei-Kumah A, Smith R, Clifton VL Cytokine, 2008;43(2):187-93. Species: Human Sample Type: Plasma Application: ELISA Development Endogenous IL-32 controls cytokine and HIV-1 production. Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA J. Immunol., 2008;181(1):557-65. Species: Human Sample Type: Cell Culture Supernates Application: Electrochemiluminescence Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F Stem Cells, 2007;26(1):279-89. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Ultrasensitive flow-based immunoassays using single-molecule counting. Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Clin. Chem., 2007;53(11):1990-5. Species: Human Sample Type: Plasma Application: ELISA Development Th2 cytokine response in Major Depressive Disorder patients before treatment. Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G J. Neuroimmunol., 2006;172(1):156-65. Species: Human Sample Type: Serum Application: ELISA Development Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray. Authors: Chen DS, Soen Y, Stuge TB PLoS Med., 2005;2(10):e265. Species: Human Sample Type: Whole Cells Application: functional microarray Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery. Authors: Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B Acta Obstet Gynecol Scand, 2005;84(6):551-7. Species: Human Sample Type: Amniotic Fluid Application: ELISA Development trans fatty acids and systemic inflammation in heart failure. Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC Am. J. Clin. Nutr., 2004;80(6):1521-5. Species: Human Sample Type: Plasma Application: ELISA Development Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. Authors: Copeland S, Siddiqui J, Remick D J. Immunol. Methods, 2004;284(1):99-106. Species: Human Sample Type: Cell Culture Supernates Application: Array Development Interplay between IFN-gamma and IL-6 signaling governs neutrophil trafficking and apoptosis during acute inflammation. Authors: McLoughlin RM, Witowski J, Robson RL, Wilkinson TS, Hurst SM, Williams AS, Williams JD, Rose-John S, Jones SA, Topley N J. Clin. Invest., 2003;112(4):598-607. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA J. Immunol., 2002;169(9):5244-51. Species: Human Sample Type: Peritoneal Fluid Application: ELISA Development The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes. Authors: Hess S, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A Arthritis Rheum., 2001;44(10):2392-401. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Peritonitis in the baboon: a primate model which stimulates human sepsis. Authors: Kinasewitz GT, Chang AC, Peer GT, Hinshaw LB, Taylor FB Shock, 2000;13(2):100-9. Species: Primate â€“ Papio cynocephalus (Baboon) Sample Type: Plasma Application: ELISA Development Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis. Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK J Korean Med Sci, 0;26(9):1132-9. Species: Human Sample Type: Serum Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-6 Pro29-Met212 Accession # P05231
生物活性	Human, Primate
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6 (5). Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (6-9). Human IL-6 is equally active on mouse and rat cells (10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 13).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Van Snick, J. (1990) Annu. Rev. Immunol. 8:253. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502. Jones, S.A. (2005) J. Immunol. 175:3468. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227. Hirano, T. et al. (1986) Nature 324:73. Alberti, L. et al. (2005) Cancer Res. 65:2. Kestler, D.P. et al. (1995) Blood 86:4559. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099. Murakami, M. et al. (1993) Science 260:1808. Muller-Newen, G. (2003) Sci. STKE 2003:PE40. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.

参考图片

IL‑6 in Human PBMCs. IL‑6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Human/Primate IL‑6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF206) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.