| 货号 | BAF1924 |
| 别名 | FLJ22252; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3 | 全称 | Transcription Factor SOX17 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human SOX17 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64321 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SOX17 Asp177-Val414 Accession # Q9H6I2 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, BAF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).
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| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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SOX17 in iBJ6 Cell Line. SOX17 was detected in immersion fixed iBJ6, an induced pluripotent stem (iPS) cell line, differentiated to endoderm using Goat Anti-Human SOX17 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
