货号 | BAF1700 |
别名 | GATA binding protein 6; GATA-binding factor 6; GATA-binding protein 6; transcription factor GATA-6 |
反应种属 | Human |
应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
目标/特异性 | Detects human GATA-6. |
使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 2627 (Human) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human GATA-6 Met1-Ala449 Accession # Q92908-2 |
生物活性 | Human |
标记 | Biotin |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | GATA-6 (also named GATA-GT1) is a zinc finger transcription activatingprotein that bins to a T/A-G-A-T-A-G/A DNA sequence motif inmesodermally and endodermally-derived tissue. There are two GATA-6isoforms that arise from alternate start sites: a short form of 449amino acids (aa), and a long form of 595 aa. The short form of humanGATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat andmouse, respectively. |
运输条件 | Blue Ice |
存放说明 | -20℃ |
参考文献 |
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GATA‑6 in KATO-III Human Cell Line. GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Human GATA‑6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |