Human Integrin alpha V Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

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Human Integrin alpha V Biotinylated Affinity Purified PAb (50 UG)

货号： BAF1219 基本售价： 5532.3 元规格： -

产品信息

概述
货号	BAF1219
别名	antigen identified by monoclonal L230; CD51 antigen; CD51; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human Integrin alpha V/CD51 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Integrin alpha V is observed, 5% cross‑reactivity with recombinant human (rh) Integrin alpha 2 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5 and rmIntegrin alpha E is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3685 (Human); 16410 (Mouse); 296456 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V/CD51 Phe31-Val992 Accession # NP_002201
生物活性	Human
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Integrin alpha V, also known as CD51 and vitronectin receptor subunit alpha, isa 140 - 150 kDa integrin alpha chain that forms dimers with at leastfive beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid(aa) residue type I membrane protein with a large (962 aa)extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. TheN-terminal region of alpha V, which is important for ligand binding,contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl)concensus repeats that fold into a beta -propellar domain. Furin cleavageof the alpha V ECD occurs after Gly 889, generating a disulfide-linked,heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECMmolecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECDof human Integrin alpha V shares 92% aa sequence identity with mouseIntegrin alpha V ECD.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Actin Stress Fibers and Focal Adhesions During EMT Adhesion Molecules Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Epithelial Cell Polarity HGF/SF Activation of Integrins Human CD Marker Products ICC / IHC Images: Integrin alpha V/CD51 Integrin Ligands at a Glance (J. Cell. Sci.) Integrin Subunit Interactions Integrins and Associated Molecules Mesenchymal Stem Cell Markers Osteoblast and Osteoclast Markers VEGF Family

参考图片

Integrin alpha V/CD51 in Human Mesenchymal Stem Cells. Integrin alpha V/CD51 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human Integrin alpha V/CD51 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.