Human Growth Hormone R Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

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Human Growth Hormone R Biotinylated Affinity Purified PAb (50 UG)

货号： BAF1210 基本售价： 5532.3 元规格： -

产品信息

概述
货号	BAF1210
别名	GH receptor; GHBP; GHR; growth hormone binding protein; growth hormone receptor; serum binding protein; Somatotropin receptor
全称	Growth Hormone Receptor
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Growth Hormone R/GHR in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse GHR and recombinant rat GHR is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2690 (Human); 14600 (Mouse); 25235 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Growth Hormone R/GHR Ala27-Tyr264 Accession # P10912
生物活性	Human
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Human GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with an 18 aa putative signal peptide, a 246 aa extracellular domain, a 24 aa transmembrane domain and a 350 aa cytoplasmic domain (5). At least two alternatively spliced isoforms of human GHR, lacking the sequence encoded by exon 3, or lacking most of the cytoplasmic domain, also exist (6, 7). Soluble GH-binding proteins corresponding to extracellular domain of the transmembrane proteins can be generated from the membrane proteins (8). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (9). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8, 10).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Goffin, V. et al. (1996) Endocrine Rev. 17:385. Le Roith, D. et al. (2001) Endocrine Rev. 22:53. Clark, R. (1997) Endocr. Rev. 18:157. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381. Leung, D.W. et al. (1987) Nature 330:537. Stallings-Mann, J.L. et al. (1996) Proc. Nat. Acad. Sci. 93:12394. Amit, T. et al. (1997) Endocr. Metab. 82:3813. Ross, R.J.M., et al. (1997) Molecular Endocrinology 11:265. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.

参考图片

Growth Hormone R/GHR in Human Breast Cancer Tissue. Growth Hormone R/GHR was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Growth Hormone R/GHR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1210) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.