货号 | BAF1177 |
别名 | Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1 | 全称 | Serpine F1/Pigment Epithelium-derived Factor |
反应种属 | Human |
应用 | Western Blot(0.1 µg/mL) |
目标/特异性 | Detects human Serpin F1/PEDF in Western blots. In this format, approximately 40% cross-reactivity with recombinant mouse Serpin F1 is observed. |
使用方法 | Western Blot: 0.1 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 5176 (Human); 20317 (Mouse); 287526 (Rat) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pigment epithelium-derived factor improves metabolic derangements and ameliorates dysregulation of adipocytokines in obese type 2 diabetic rats. | |
纯化方式 | Antigen Affinity-purified |
免疫原 | recombinant human Serpin F1/PEDF |
生物活性 | Human |
标记 | Biotin |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | Serpin F1 (serineproteinaseinhibitor-cladeF #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes. Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate. Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity. Human serpin F1 is 418 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387-411). Phosphorylation occurs at multiple sites that affect bioactivity. Over aa 44-418, human serpin F1 shares 87% aa identity with mouse serpin F1. |
运输条件 | Blue Ice |
存放说明 | -20℃ |
参考文献 |
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