概述| 货号 | BAF1159 |
| 别名 | IEGFMSTP036; Iris-expressed growth factor; PDGF-D; platelet derived growth factor D; SCDGF-BMGC26867; SCDGFBplatelet-derived growth factor D; spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal cord-derived growth factor-B |
全称 | Platelet-derived Growth Factor D | | 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL)
|
| 目标/特异性 | Detects human PDGF-D in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) PDGF-AA, rhPDGF-AB and rhPDGF-BB is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL
|
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 80310 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PDGF-D |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The platelet-derived growth factor (PDGF) family consists of four disulfide-linked homodimers and one heterodimer (PDGF-AB). These proteins regulate diverse cellular functions through interactions with PDGF R alpha and R beta (1, 2). Mature PDGF-DD associates with PDGF R beta and triggers signaling through PDGF R beta homodimers and PDGF R alpha / beta heterodimers (3 - 5). The human PDGF-DD cDNA encodes a 370 amino acid (aa) precursor that includes a 23 aa signal sequence, one CUB domain, and one PDGF/VEGF domain (3, 4). The PDGF/VEGF domain shares 27 - 35% aa sequence identity with the corresponding regions of other PDGF family members. Human PDGF-DD shares 87% aa sequence identity with mouse and rat PDGF-DD. PDGF-DD is secreted as a100 kDa latent homodimer which is activated by proteolysis to release a 35 kDa bioactive protein containing the PDGF/VEGF homology domain (3,4,6,7). A splice variant of PDGF-DD has a 6 aa deletion near the N-terminus. A 72 aa deletion within the PDGF/VEGF domain generates an inactive protein in mouse but has not been detected in human (8). PDGF-DD is widely expressed in embryonic and adult tissues (3, 9, 10), and PDGF R beta is expressed in a generally complementary pattern (9, 11, 12). PDGF-DD functions as a growth factor for renal artery smooth muscle cells and lens epithelial cells, and as a macrophage chemoattractant (5, 9 - 11). PDGF-DD is overexpressed in and contributes to several disease states, including renal and hepatic fibrosis, mesangial proliferative glomerulopathy, pulmonary lymphoid infiltration, and many cancers (6, 11 - 15). PDGF-DD functions in both paracrine and autocrine manners (6, 7, 14). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Reigstad, L.J. et al. (2005) FEBS J. 272:5723.
- Fredriksson, L. et al. (2004) Cytokine Growth Factor Rev. 15:197.
- LaRochelle, W.J. et al. (2001) Nat. Cell Biol. 3:517.
- Bergsten, E. et al. (2001) Nat. Cell Biol. 3:512.
- Uutela, M. et al. (2004) Blood 104:3198.
- Ustach, C.V. and H-R.C. Kim (2005) Mol. Cell. Biol. 25:6279.
- Ustach, C.V. et al. (2004) Canc. Res. 64:1722.
- Zhuo, Y. et al. (2003) Biochem. Biophys. Res. Commun. 308:126.
- Changsirikulchai, S. et al. (2002) Kid. Int. 62:2043.
- Ray, S. et al. (2005) J. Biol. Chem. 280:8494.
- Hudkins, K.L. et al. (2004) J. Am. Soc. Nephrol. 15:286.
- Lokker, N.A. et al. (2002) Canc. Res. 62:3729.
- Taneda, S. et al. (2003) J. Am. Soc. Nephrol. 14:2544.
- LaRochelle, W.J. et al. (2002) Canc. Res. 62:2468.
- Xu, L. et al. (2005) Canc. Res. 65:5711.
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