概述| 货号 | PPS057 |
| 别名 | NMDA R, NR2B Subunit; NMDAR2B; NR2B |
全称 | Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B | | 应用 | Western Blot(1:1000 dilution)
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| 目标/特异性 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 使用方法 | Western Blot: 1:1000 dilution
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| 来源 | Polyclonal Rabbit IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2904 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1336 of NMDA R, NR2B Subunit |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 背景 | NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics and mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The NR2B subunit generates a high conductance NMDAR. Human NR2B (also NR3;GRIN2B and GluRe2) is a 180-200 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD), and a 646 aa cytoplasmic region. At least three tyrosines are known to be phosphorylated by Fyn in the cytoplasmic region. These include Y1252, Y1336 and Y1472. While much is known about Y1472, little information is available about the consequences of Y1252 and Y1336 phosphorylation. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
- Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
- Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
- Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
- Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.
- Nakazawa, T. et al. (2001) J. Biol. Chem. 276:693.
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参考图片Detects Phospho-GRIN2B/NMDAR2B (Y1336) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1336 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l PPase). The blot is identical to the control except that it was incubated in l PPase (1200 units for 30 minutes) before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1336). The immunolabeling is completely eliminated by treatment with l PPase. |
