概述| 货号 | PPS058 |
| 别名 | PAK |
全称 | p21/Cdc42/Rac1-activated Kinase 1, 2, 3 | | 应用 | Western Blot(1:1000 dilution)
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| 目标/特异性 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 使用方法 | Western Blot: 1:1000 dilution
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| 来源 | Polyclonal Rabbit IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-T402 of PAK |
| 生物活性 | Human, Mouse, Rat, Primate |
| 标记 | Unconjugated |
| 背景 | PAK (p21-activated kinase) is a general term for a Serine/Threonine kinase that functions downstream of Rho-family GTPases to regulate cytoskeletal (actin) structure and gene transcription. There are two mammalian PAK groups, each with three members. Group I contains PAK-1/ alpha -PAK, PAK-2/ beta -PAK and PAK-3/ gamma -PAK; group II contains PAKs 4-6. All PAKs contain p21-binding domains and a kinase domain. Only group I PAKs are activated by Cdc42 (cell division cycle 24) and exist as dimers. The dimeric form of group I PAKs keep them in an inactive state. This is due to cross-inhibition from one monomer to another. Following p21 binding, these PAKs dissociate and undergo either auto-, or exogenous (PKD1) phosphorylation on a kinase domain Threonine that is embedded in an 18 aa sequence that is absolutely conserved in PAKs 1-3 in both rat and human. In rat, this Threonine occurs at 402 in PAK-2, T422 in PAK-1, and T421 in PAK-3. In human, each position is increased by one (i.e. T403 vs. T402 in rat PAK-2, etc.). Phosphorylation at this homologous site activates the PAKs, which, following additional regulatory phosphorylations, can then interact with desmin, myosin light chain kinase, LIM1, filamin A and/or Merlin. Preparation Prepared from rabbit serum by affinity purification via sequential chromatography |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Bokoch, G.M. (2003) Annu. Rev. Biochem. 72:743.
- Zkumar, R. et al. (2006) Nat. Rev. Cancer 6:459.
- Parrini, M.C. et al. (2002) Mol. Cell 9:73.
- King, C.C. et al. (2000) J. Biol. Chem. 275:41201.
- Chong, C. et al. (2001) J. Biol. Chem. 276:17347.
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参考图片Detection of Phospho-PAK (T402) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately
68-70 kDa PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the anti-Phospho-PAK-1, 2, 3 (T402). The immunolabeling of PAK is completely eliminated by treatment with lambda PPase. |
