Phospho-Synapsin I (S9) Affinity Purified Polyclonal Ab (100 UL)
货号：
PPS084 基本售价：
5373.6 元 规格：
-
产品信息
概述| 货号 | PPS084 |
| 别名 | SYN1; SYN1a; SYN1b; Synapsin 1; synapsin IBrain protein 4.1; synapsin-1; SYNI |
| 应用 | Western Blot(1:1000 dilution)
|
| 目标/特异性 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 使用方法 | Western Blot: 1:1000 dilution
|
| 来源 | Polyclonal Rabbit IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 6853 (Human); 20964 (Mouse); 24949 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S9 of Synapsin I |
| 生物活性 | Human, Mouse, Rat, Xenopus |
| 标记 | Unconjugated |
| 背景 | Synapsin I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release. It is concentrated in presynaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton. Phosphorylation at various sites reduces synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release. Rat synapsin I is 704 amino acids (aa) in length and contains five domains, named A - E/F. Domains A - D lie between aa 1 - 655, while E and F are alternate splice forms. Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner. Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation. Phosphorylation of S603 and 566 in domain D, and S9 in domain A, reduces the affinity of synapsin I for actin and secretory vesicles. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Yamagata, Y. (2003) J. Pharmacol. Sci. 93:22.
- Sakurada, K. et al. (2002) J. Biol. Chem. 277:45473.
- Hilfiker, S. et al. (2005) J. Neurosci. 25:2658.
- McCaffery, C.A. and L.J. DeGennaro (1986) EMBO J. 5:3167.
- Cole, R.N. and G.W. Hart (1999) J. Neurochem. 73:418.
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参考图片Detection of Phospho-Synapsin I (S9) by Western Blot. Western blot of rat brain lysate showing specific immunolabeling of the approximately 78 kDa Synapsin I phosphorylated at S9 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the anti-phospho-S9 Synapsin I. The immunolabeling is completely eliminated by treatment with lambda PPase. |
