| 货号 | PPS073 |
| 别名 | G protein-coupled receptor 51; GABA-BR2; GABA-B-R2; GABABR2GABA-B receptor 2; GABBR2; gamma-aminobutyric acid (GABA) B receptor, 2; gamma-aminobutyric acid type B receptor subunit 2; gb2; GPR51; GPR51GABA-B receptor, R2 subunit; GPRC3B; GPRC3BFLJ36928; G-protein coupled receptor 51; HG20HRIHFB2099 | 全称 | gamma-Aminobutyric Acid Type B Receptor Subunit 2 |
| 应用 | Western Blot(1:1000 dilution) Immunofluorescence(1:100 - 1:250 dilution) |
| 目标/特异性 | Human, mouse, rat, and Xenopus ~110 kDa GABAB Receptor, R2 subunit phosphorylated at S892 |
| 使用方法 | Western Blot: 1:1000 dilution Immunofluorescence: 1:100 - 1:250 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9568 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S892 of GABABR2 |
| 生物活性 | Human, Mouse, Rat, Xenopus |
| 标记 | Unconjugated |
| 背景 | The GABA-B ( gamma -aminobutyric acid-type B) receptors (GABABR) are C family members of the GPCR superfamily. There are only two family members, GABARB1(a & b) and GABARB2, which form a nondisulfide-linked heterodimeric receptor in the native state. GABARB heterodimers are found both pre- and post-synaptically, where they modulate glutamate release presynaptically, and prolong neuronal hyperpolarization postsynaptically. Rat GABARB2 is a 110 kDa, 900 amino acid (aa), 7-transmembrane glycoprotein. It has an extended, 442 aa N-terminal extracellular region that contains the GABA binding site. Unlike most other GPCRs, PKA phosphorylation of the GABAB2 cytoplasmic tail does not promote GABAB2 internalization. To the contrary, phosphorylation of S892 promotes surface expression, while dephosphorylation promotes internalization. Across species (human to frog to mouse to rat), there is 88% aa identity in the 200 aa cytoplasmic tail, and 85% aa identity over the entire mature receptor. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Phospho-GABAB R2 (S892) by Western Blot Western blots of COS-7 cells transiently expressing mutant GABAB R2 (S892A) (lane 1), wild type GABABR2 (lane 2) or control untransfected cells (lane 3) were lysed and 50 mg of protein from each sample was separated by SDS-PAGE and transferred to a nitrocellulose membrane. Blots were then immunolabeled with rabbit anti-phospho-GABAB R2 (S892) (A). In B, the membrane was pre-treated with lambda -PPase (1200 units for 30 minutes) before being exposed to the anti-GABAB Receptor, R2 subunit (S892). The immunolabeling is completely eliminated by treatment with lambda -PPase. |
