| 货号 | PPS074 |
| 别名 | alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFNBR; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1 | 全称 | Interferon alpha/beta Receptor 1 |
| 应用 | Western Blot() Immunohistochemistry(1:1000 dilution) |
| 目标/特异性 | Human IFN-alpha R1, Type I, Subunit I phosphorylated at S535/S539 |
| 使用方法 | Western Blot: 1:1000 dilution HEK293 human embryonic kidney cell line transfected with IFN-alpha R1 Note: The molecular weight of the IFN-alpha R1 varies with cell line (different levels of glycosylation) in HEK293 and HeLa cells; the mature form is ~110 - 130 kDa. Immunohistochemistry: 1:1000 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3454 (Human); 15975 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S535/S539 of IFN-alpha R1 Subunit I |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (1,2). These cytokines produce their effects by binding to the Type I IFN Receptor. This receptor contains two subunits; IFNAR1/ alpha 1 and IFNAR2/ alpha 2 Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This downregulation is thought to be influenced by phosphorylation of S535 and S539 in IFN-alpha R1 (3). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Phospho-IFN-alpha / beta R1 (S535/S539) by Western Blot Western blot of immunoprecipitates from HEK293 cells transfected with 1) Mock, 2) IFN-alpha R1 wild type, and 3) IFN-alpha R1 S535A and S539A mutants showing specific immunolabeling of the ~110 kDa to ~130 kDa IFN-alpha R1 wild type. The immunolabeling is absent in the IFN-alpha R1 S535 and S539 mutants (Control). The immunolableing is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos). |
