Phospho-5-Lipoxygenase (S523) Affinity Purified PAb (100 UL)
货号：
PPS001 基本售价：
5373.6 元 规格：
-
产品信息
概述| 货号 | PPS001 |
| 别名 | 5-lipoxygenase; 5-LO; 5-LOX; 5LPG; ALOX5; arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; EC 1.13.11; EC 1.13.11.34; leukotriene A4 synthase; LOG5; LOX5A; MGC163204 |
| 应用 | Western Blot(1:1000 dilution)
|
| 目标/特异性 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 使用方法 | Western Blot: 1:1000 dilution
|
| 来源 | Polyclonal Rabbit IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 240 (Human); 11689 (Mouse); 25290 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S523 of human 5-Lipoxygenase (5-LO) |
| 生物活性 | Human, Rat, Primate |
| 标记 | Unconjugated |
| 背景 | 5-Lipoxygenase (5-LO) is an 80 kDa, 676 amino acid (aa) nonheme iron dioxygenase. It converts nuclear membrane arachidonic acid into leukotriene A4 (LTA4) that is then modified to produce either LTB4 or LTC4. 5-LO is found in leukocytes and generally exists in the cytoplasm. Upon activation, 5-LO will either remain cytosolic or undergo translocation into the nucleus. In the nucleus, its enzyme products impact gene expression; in the cytosol, its products are secreted and contribute to inflammation. 5-LO has three Nuclear Localization Signal (NLS) motifs starting at amino acids 112, 158, and 518. Upon cell activation, Ser at 523 is phosphorylated by Protein Kinase A (PKA). This amino acid lies within the principal NLS of 5-LO. When phosphorylated, the NLS is inactivated, blocking 5-LO entry into the nucleus. This appears to impact overall leukotriene biosynthesis, and potentially serves as a regulatory mechanism for inflammation. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Jones, S.M. et al. (2003) J. Biol. Chem.278:10257.
- Dixon, R.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:416.
- Luo, M. et al. (2005) J. Biol. Chem. 280:40609.
- Funk, C. (2001) Science 294:1871.
|
参考图片Detection of Phospho-5‑Lipoxygenase (S523) by Western Blot. Western blot of rat brain (cortex) tissue lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at S523 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-5-Lipoxygenase (S523). The immunolabeling is completely eliminated by treatment with lambda -PPase. |
