| 货号 | PPS064 |
| 别名 | activating transcription factor 2formerly; cAMP responsive element binding protein 2; cAMP-responsive element-binding protein 2; CREB2; CREBP1; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; HB16; MGC111558; mXBP; TREB7 | 全称 | Activating Transcription Factor 2 |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1:1000 dilution) Immunohistochemistry(1:1000 dilution) |
| 目标/特异性 | Human and rat ~74 kDa and ~54 kDa splice form of ATF2 phosphorylated at S490/S498 in Western blots. |
| 使用方法 | Western Blot: 1:1000 dilution Immunohistochemistry: 1:1000 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1386 (Human); 11909 (Mouse); 81647 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S490/S498 of ATF2 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 背景 | ATF2 (activating transcription factor 2; also CRE-BP1) is a 60 kDa member of the ATF subfamily, basic-region leucine zipper family of DNA binding proteins. It has at least two activities. First, it binds to an octameric palindromic CRE (cAMP response element) 5’-TGACGTCA-3’, and secondary, it shows histone acetyltransferase (HAT) activity towards histones H2B and H4. Its palindromic binding activity depends upon homodimerization, or heterodimerization with either c-Jun or CRE-Bpa. Human ATF2 is 505 amino acids (aa) in length, and contains a C2H2-type zinc finger segment (aa 25-49), a HAT region (aa 112-350), and a basic leucine zipper domain (aa 350-414). There are over a dozen alternate splice forms that, in their entirety, impact all segments of the molecule. ATF2 is phosphorylated at multiple sites, including S62/S90/S112/S340/S368/S490/S498 and T69/T71. The Thr phosphorylations increase transcriptional and HAT activity. Serine phosphorylations at S490/S498 by ATM contribute to DNA damage repair after ionizing radiation. Human ATF2 is greater that 95% aa identical to mouse ATF2 and 100% identical to rat ATF2. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|