Phospho-PLKK (S482/486/490) Affinity Purified Polyclonal Ab (100 UL)
货号：
PPS034 基本售价：
5373.6 元 规格：
-
产品信息
概述| 货号 | PPS034 |
| 别名 | PLKK |
全称 | Polo-like Kinase Kinase | | 应用 | Western Blot(1:1000 dilution)
|
| 目标/特异性 | Human, mouse, rat, zebrafish, and Xenopus ~120 kDa PLKK |
| 使用方法 | Western Blot: 1:1000 dilution
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 5347 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S482/486/490 of XenopusPLKK |
| 生物活性 | Human, Mouse, Rat, Xenopus, Zebrafish |
| 标记 | Unconjugated |
| 背景 | Xenopus Polo-Like Kinase Kinase (xPLKK1) is a 120 kDa Ser/Thr kinase that is associated with 67 kDa PLx1 (Polo-Like Xenopus Kinase 1). PLx1 is involved with spindle assembly and centrosome maturation during the late G2 and M cell cycle phase. A cell’s entry into mitosis is controlled by the activation of Cyclin B/cdc2. cdc2 is constitutively phosphorylated and inactive, and activated by dephosphorylation of Tyr at position 15. The phosphatase that accomplishes this is cdc25C, which is activated by phosphorylation at multiple sites by a polo-like kinase termed PLx1 (PLK1 in mammals). The activating enzyme that acts on PLx1 is under investigation, and the kinase termed xPLKK1 has been identified as a candidate. xPLKK1 is 950 amino acids (aa) in size and contains a 264 amino acid kinase domain (aa 31 - 294). It contains three serines within a nine amino acid span (S482/486/490) which, when phosphorylated, activate the enzyme. Activation of PLx1 appears to depend on the phosphorylation of T201. And while xPLKK1 phosphorylates PLx1, it does so only on T10. Thus, its role in PLx1 activation is unclear. Notably, PLx1 has been shown to activate xPLKK1 on S482/486/490, suggesting that xPLKK1 may actually be a downstream target of PLx1 rather than an activator of PLx1. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Lowery, D.M. et al. (2004) Cell Cycle 3:128.
- Erickson, E. et al. (2004) J. Biol. Chem. 279:32219.
- Qian, Y-W. et al. (1998) Science 282:1701.
- Kelm, O. et al. (2002) J. Biol. Chem. 277:25247.
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