概述| 货号 | PPS006 |
| 别名 | Axonal membrane protein GAP-43; B-50; Basp2; calmodulin-binding protein P-57; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; PP46; protein F1 |
全称 | Growth Associated Protein 43 | | 应用 | Western Blot(1:1000 dilution)
|
| 目标/特异性 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 使用方法 | Western Blot: 1:1000 dilution
|
| 来源 | Polyclonal Rabbit IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2596 (Human); 14432 (Mouse); 29423 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S41 of GAP-43 |
| 生物活性 | Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Xenopus, Zebrafish |
| 标记 | Unconjugated |
| 背景 | GAP-43 (Growth Associated Protein 43 kDa), also known as Neuromodulin, is a 24 kDa, neuron growth cone-associated member of the neuromodulin family. Apparently due to its tertiary structure, it runs anomalously at 43 kDa on SDS-polyacrylamide gel. Rat GAP-43 is a 226 amino acid (aa) protein that contains an N-terminal lipid binding motif, an 11 aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa 38 - 48), and a C-terminus with four potential serine phosphorylation sites. The molecule may utilize the IQ domain to bind calmodulin. Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction. Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Axon Guidance and Neuron Migration
|
参考图片Detection of Phospho-GAP‑43 (S41) by Western blot. Western blot of rat cortex lysate showing specific immunolabeling of the ~50 kDa GAP-43 protein phosphorylated at S41 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-GAP-43 (S41). The immunolabeling is completely eliminated by treatment with lambda -PPase. |
