Human/Mouse/Rat Phospho-Elk-1 (S383) Affinity Purified PAb (100 UL)
货号：
PPS021 基本售价：
5373.6 元 规格：
-
产品信息
概述| 货号 | PPS021 |
| 别名 | ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS-like gene 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1:1000 dilution)
|
| 目标/特异性 | Human, mouse and rat ~46 kDa Elk-1 protein phosphorylated at S383 |
| 使用方法 | Western Blot: 1:1000 dilution
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2002 (Human); 13712 (Mouse); 314436 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S383 of Elk-1 |
| 生物活性 | Human, Mouse, Rat, Canine, Primate, Zebrafish |
| 标记 | Unconjugated |
| 背景 | Elk-1 (ETS-like protein 1) is a 45 kDa member of the ETS family of serum response element-binding transcription factors. It is 428 amino acids (aa) in length and contains an N-terminal ETS DNA-binding domain (aa 3 - 86), a serum response factor (SRF) binding segment (B box) (aa 148 - 168), a MAPK docking site (D domain) (aa 310 - 334), and a C-terminal transactivation domain (C domain) (aa 336 - 428). Elk-1 is normally SUMOlyated on R230, R249 and R254. This interferes with nuclear localization and activation. It is also bound to p300 in an inactive complex. Phosphorylation by MAPK1 at S383 and S389 removes SUMO protein, allowing for nuclear localization, and activation of the pre-existing p300/Elk-1 complex that is capable of rapidly initiating gene transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Yang, S-H. et al. (2003) Mol. Cell 12:63.
- Rao, V.N. et al. (1989) Science 244:66.
- Li, Q-J. et al. (2003) EMBO J. 22:281.
- Salinas, S. et al. (2004) J. Cell Biol. 165:767.
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参考图片Detection of Phospho-Elk-1 (S383) by Western Blot Western blot of phosphorylated and unphosphorylated recombinant Elk-1 protein showing phospho-specific immunolabeling of the ~46 kDa Elk-1 protein. |
