| 货号 | PPS088 |
| 别名 | DYT14; DYT5b; EC 1.14.16; EC 1.14.16.2; TH; TYH dystonia 14; TYH; Tyrosine 3-hydroxylase; tyrosine 3-monooxygenase; tyrosine hydroxylase |
| 应用 | Western Blot(1:1000 dilution) Immunohistochemistry(1:1000 dilution) Immunofluorescence(1:1000 dilution) |
| 目标/特异性 | Mouse and rat ~62 kDa Tyrosine Hydroxylase phosphorylated at S31 |
| 使用方法 | Western Blot: 1:1000 dilution Immunohistochemistry: 1:1000 dilution Immunofluorescence: 1:1000 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7054 (Human); 21823 (Mouse); 25085 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cyclin-dependent kinase 5 in the ventral tegmental area regulates depression-related behaviors. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S31 of Tyrosine Hydroxylase |
| 生物活性 | Mouse, Rat, Primate |
| 标记 | Unconjugated |
| 背景 | Rat tyrosine hydroxylase (TH) is a pterin-dependent monooxygenase that catalyzes the hydroxylation of tyrosine to DOPA through the use of a non-heme iron. It is a 55 kDa, 498 amino acid (aa) alpha -helical protein that runs anomalously at 62 kDa in SDS-PAGE. The molecule contains a 164 aa N-terminal regulatory region and a 334 aa C-terminal catalytic domain. Its activity is regulated at the post-transcriptional level by phosphorylation of serine and feedback by catecholamines. Four serines are known to be phosphorylated at aa positions 8, 19, 31, and 40. Different kinases contribute to different phosphorylation patterns. For example, MAPKAPK-2 and CaMKII act on S19 and S40, PKA phosphorylates only at S40, while Cdk5 phosphorylates S31. Variable site phosphorylations have variable effects. S40 phosphorylation blocks catecholamine feedback inhibition. Subsequent phosphorylations at S19 or S31 likely stabilize an otherwise unstable enzyme phosphorylated only at S40, contributing to increased enzyme activity. Phosphorylation at S8 is likely to be physiologically unimportant. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Phospho-Tyrosine Hydroxylase (S31) by Western Blot Western blot of PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 μM for 60 minutes) showing specific immunolabeling of the approximately 62 kDa Tyrosine Hydroxylase phosphorylated at S31. |
