概述| 货号 | PPS010 |
| 别名 | 80K-L protein; 80K-L; FLJ14368; MACS; MACSmyristoylated alanine-rich C-kinase substrate; MRACKS; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L); myristoylated alanine-rich protein kinase C substrate; phosphomyristin; PKCSLFLJ90045; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain |
全称 | Myristoylated Alanine-rich Protein Kinase C Substrate | | 应用 | Western Blot(1:1000 dilution)
|
| 目标/特异性 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 使用方法 | Western Blot: 1:1000 dilution
|
| 来源 | Polyclonal Rabbit IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 4082 (Human); 17118 (Mouse); 25603 (Rat) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release.
Authors: Obis T, Besalduch N, Hurtado E, Nadal L, Santafe M, Garcia N, Tomas M, Priego M, Lanuza M, Tomas J
Mol Brain, 2015;8(0):8.
Species: Rat
Sample Type: Whole Tissue
Application: WB |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S152/S156 of MARCKS |
| 生物活性 | Human, Mouse, Rat, Bovine, Chicken, Xenopus, Zebrafish |
| 标记 | Unconjugated |
| 背景 | Rat MARCKS (Myristoylated, Alanine-rich C Kinase protein Substrate) is an 83 kDa, 309 amino acid intracellular protein that belongs to the MARCKS family of molecules. It is an actin-organizing molecule and a key substrate for activated protein kinase C (PKC) and PKC-related kinase. MARCKS is normally sequestered on the inner face of the plasma membrane. Here, it is bound to phosphatidylserine (PS) via electrostatic and hydrophobic interactions. Upon integrin ligation ( alpha 5 beta 1), PKC is activated, and phosphorylates MARCKS at S152 and S156. This breaks the PS interaction, forcing MARCKS into the cytosol. Subsequent cytosolic dephosphorylation of MARCKS induces MARCKS-mediated polymerization of actin and its return to the membrane. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | |
参考图片Detection of Phospho-MARCKS (S152/S156) by Western Blot. Western blot of rat brain lysate showing specific immunolabeling of the ~83 kDa MARCKS protein phosphorylated at S152/S156 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-MARCKS (S152/S156). The immunolabeling is completely eliminated by treatment with lambda -PPase. |
