Phospho-Synuclein-alpha (S129) Affinity Purified PAb (100 UL)
货号：
PPS091 基本售价：
5373.6 元 规格：
-
产品信息
概述| 货号 | PPS091 |
| 别名 | Lewy body) 4; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; non-A4 component of amyloid; PARK1; PARK4; PD1; SNCA; synuclein, alpha (non A4 component of amyloid precursor) |
| 应用 | Western Blot(1:1000 dilution)
|
| 目标/特异性 | Human, mouse, rat, bovine, canine, and non-human primate Synuclein-alpha phosphorylated at S129 |
| 使用方法 | Western Blot: 1:1000 dilution
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding the phospho-S129 of Synuclein-alpha |
| 生物活性 | Human, Mouse, Rat, Bovine, Canine, Primate |
| 标记 | Unconjugated |
| 背景 | Synuclein-alpha is a 14 kDa member of the synuclein family. It is found in both the neuron nucleus and the cytosol of presynaptic nerve terminals in the brain. Synuclein-alpha is 140 amino acids in length and runs anomalously at 19 kDa in SDS-page. It contains three domains; an N-terminal lipid-binding domain, a central amyloid-binding region, and a C-terminal acidic tail. The N-terminal area (aa 1 - 100) is involved with lipid (membrane) and protein binding. The C-terminus may be regulatory. There is a NAC (non-Ab component of AD amyloid) segment between aa 61 - 95. This has been thought to mediate synuclein-alpha filament formation and microtubial stabilization. Whether it exists as a stand-alone normal cleavage product of synuclein-alpha is unclear. Synuclein-alpha is phosphorylated on multiple sites. S129 undergoes constitutive phosphorylation and dephosphorylation. When phosphorylated, filament formation (and perhaps oligomerization) is promoted. Uncontrolled filament/fibril formation is suggested to be involved in Parkinson’s disease Lewy body formation. Tyrosine phosphorylation also occurs at Y125. Synuclein-alpha is known to bind to, and inhibit, PLD-1 and -2. When phosphorylated at Y125, synuclein-alpha activity is decreased and PLD activity is increased. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - da Costa, C.A. (2003) Curr. Mol. Med. 3:17.
- Ueda, K. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11282.
- Okochi, M. et al. (2000) J. Biol. Chem. 275:390.
- Goers, J. et al. (2003) Biochemistry 42:8465.
- Ahn, B-H. et al. (2002) J. Biol. Chem. 277:12334.
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参考图片Detection of Phospho-Synuclein-alpha (S129) by Western Blot Western Blot of rat cortex lysate showing specific labeling of the ~15 kDa Synuclein-alpha protein phosphorylated at S129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown). |
