| 货号 | PPS009 |
| 别名 | Glycine R |
| 反应种属 | Rat |
| 应用 | Western Blot(1:1000 dilution) Immunohistochemistry(1:1000 dilution) |
| 目标/特异性 | Specific for the ~48 kDa alpha 1 and alpha 2 subunits of the Glycine Receptor in Western blots of rat spinal cord, brain stem and cell extracts. Immunolabeling is blocked by preadsorption of antibody with the peptide immunogen. This antibody does not recognize other Glycine Receptor subunits. |
| 使用方法 | Western Blot: 1:1000 dilution Immunohistochemistry: 1:1000 dilution |
| 来源 | This antibody should be reconstituted in 100 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Peptide from the N-terminus region of the alpha 1 subunit of the rat Glycine Receptor |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | This antibody should be reconstituted in 100 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. |
| 背景 | The rat glycine receptor (GlyR) chloride channel is a member of the nicotinic acetylcho-line receptor family of ligand-gated ion channels. Its glycine-mediated activation results in an influx of chlorine ions that hyperpolarize and desensitize neuronal postsynaptic membranes. The receptor’s multimeric makeup is unclear. It may be either a three or five subunit homo- or heteromultimer. To date, there are five rat GlyR subunit types. Four are 48-53 kDa alpha -type subunits, and one is a 58 kDa beta -type subunit. Alternate splicing exists for alpha 1 and alpha 2 subunit types. Alpha-type subunits may form homotrimers; however, their function may not be neurotransmission but rather non-synaptic cell-cell communication. Alpha1-beta multimers may be the principal synaptic GlyR in adults. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Rat Glycine R by Western Blot. Western blot of 10 μg of rat spinal cord. As shown in the autoradiograph, the Anti-Glycine Receptor antibody is specific for the ~48 kDa subunits recognizing both the alpha 1 and alpha 2 subunits of the Glycine Receptor. |
