概述| 货号 | PPS078 |
| 别名 | GLUR2; glutamate metabotropic receptor 2; glutamate receptor, metabotropic 2; GPRC1Bmetabotropic glutamate receptor 2; mGlu2; mGluR2; MGLUR2glutamate receptor homolog |
全称 | Metabotropic Glutamate Receptor 2 and 3 | | 反应种属 | Rat |
| 应用 | Western Blot(1:1000 dilution)
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| 目标/特异性 | Rat ~110 kDa mGluR2 and mGluR3 monomers and ~220 kDa homodimers |
| 使用方法 | Western Blot: 1:1000 dilution
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. mGluR5 in the nucleus accumbens is critical for promoting resilience to chronic stress.
Authors: Shin S, Kwon O, Kang J, Kwon S, Oh S, Choi J, Kim C, Kim D
Nat Neurosci, 2015;18(7):1017-24.
Species: Mouse
Sample Type: Tissue Lysate
Application: WB |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Peptide from the C-terminus region of rat mGluR2 and mGluR3 |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 背景 | Rat metabotropic glutamate receptor 2 (mGluR2; also mGlu2) is a 95 kDa, 854 amino acid (aa), 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. Group II receptors include mGluR2 and mGluR3. Both are presynaptic, considered to negatively regulate the release of glutamate, associate with Gi/Go proteins, and inhibit adenylate cyclase, thus decreasing cAMP concentration. The effects of mGluR2 and 3 are not always identical. On microglia, mGluR2 but not mGluR3 stimulation induces TNF-alpha release. mGluR2 is believed to exist as a dimer. Dimerization presumably creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. The cytoplasmic tails of rat mGluR2 and mGluR3 are 64% aa identical; over the last 18 aa, they are 95% aa identical. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Rudd, M.T. and J.A. Mccauley (2005) Curr. Top. Med. Chem. 5:869.
- Gasparini, F. et al. (2002) Curr. Opin. Pharmacol. 2:43.
- Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
- Shen, K-Z. and S.W. Johnson (2003) J. Physiol. 533:489.
- Taylor, D.L. et al. (2005) J. Neurosci. 25:2952.
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参考图片Detection of mGluR2/3 by Western Blot Western blot of rat hippocampal lysate showing the specific immunolabeling of the ~110 kDa monomer and the ~220 kDa dimer of mGluR2 and mGluR3. |
