概述| 货号 | PPS077 |
| 别名 | glutamate receptor, metabotropic 1; GPRC1A; GRM1; GRM1A; metabotropic glutamate receptor 1; mGluR1; MGLUR1A; MGLUR1GPRC1AmGlu1 |
全称 | Metabotropic Glutamate Receptor 1 | | 反应种属 | Rat |
| 应用 | Western Blot(1:1000 dilution)
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| 目标/特异性 | Rat ~125 kDa and ~250 kDa dimer mGluR1a |
| 使用方法 | Western Blot: 1:1000 dilution
Immunohistochemistry: (fozen sections; unpublished observations)
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| 来源 | Polyclonal Rabbit IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2911 (Human); 14816 (Mouse); 24414 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Peptide from the C-terminus region of rat mGluR1a |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 背景 | Rat metabotropic glutamate receptor 1 (mGluR1a; also mGlu1a) is a 140 kDa, 1181 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. When co-expressed on select neurons, mGluR5 tends to dampen mGluR1 activity. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks proliferation. mGluR1 has a large glycosylated extracellular domain (ECD) of 574 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There are two alternate splice forms of rat mGluR1. Each shows a short aa substitution and premature truncation after N887 in the cytoplasmic region. The cytoplasmic, C-terminal 310 amino acids of rat mGluR1a are 90% and 97% aa identical to the analogous amino acids in human and mouse mGluR1a, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Pin, J-P. and F. Acher (2002) Curr. Drug Targets CNS Neurol. Disord. 1:297.
- Boldyrev, A.A. et al. (2005) J. Neurochem. 95:913.
- Poisik, O.V. et al. (2003) J. Neurosci. 23:122.
- Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
- Gama, L. et al. (2001) J. Biol. Chem. 276:39053.
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参考图片Detection of mGluR1 C-Terminus by Western Blot Western blot of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimer of mGluR1a. |
