| 货号 | PPS069 |
| 别名 | DA transporter; DAT1; DATDAT1Solute carrier family 6 member 3; SLC6A3; sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; VNTR | 全称 | Dopamine Transporter |
| 应用 | Western Blot(1:1000 dilution) Immunohistochemistry(1:1000 dilution) |
| 目标/特异性 | Specific for the ~88 kDa DAT protein in Western blots of SDS-solubilized human striatal samples and in IHC applications with formaldehyde-fixed human and monkey brain sections. |
| 使用方法 | Western Blot: 1:1000 dilution Immunohistochemistry: 1:1000 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6531 (Human); 13162 (Mouse); 24898 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | SLC6A3/DAT1 |
| 生物活性 | Human, Primate |
| 标记 | Unconjugated |
| 背景 | The dopamine transporter (DAT) is a 70-80 kDa member of the Na+-neurotransmitter symporter family of transmembrane (TM) proteins. DAT plays a crucial role in the synaptic clearance of dopamine (DA). It mediates the uptake of dopamine by the presynaptic terminal, thus limiting the strength of the dopamingeric response. One molecule of DA is accompanied by two Na+ and one Cl- ion. Molecules such as amphetamine both competitively inhibit DA uptake, and induce DA release through the DAT, increasing the rewarding property of DA. Human DAT is a 620 amino acid (aa), 12 TM phosphoglycoprotein with an N- and C-terminal cytoplasmic domain. It exists as a disulfide-linked oligomer on the cell surface. Phosphorylation of the N-terminus (S7/12) promotes DA release. The C-terminus binds CaMKII, as well as Hic5, Pick1, and synuclein which regulate receptor trafficking and expression. There is an extended 71 aa extracellular (EC) loop between TM segments 3 and 4. Glycosylation at this site is necessary for oligomer expression on the cell membrane. The human 42 aa DAT C-terminus is 93% aa identical to the mouse DAT C-terminus. The human EC loop is also 93% aa identical to the rhesus monkey EC loop. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of SLC6A3/DAT1 (Extracellular Loop 2) by Western Blot. Western blot of SDS-solubilized human caudate (20 μg protein) showing the ~88 kDa band representing the DAT protein. |
