| 货号 | PPS067 |
| 别名 | DBH; DBM; dopamine beta-hydroxylase (dopamine beta-monooxygenase); dopamine beta-hydroxylase; Dopamine beta-monooxygenase; DOPBHY; EC 1.14.17.1 |
| 应用 | Western Blot(1:1000 dilution) |
| 目标/特异性 | Human and monkey ~75 kDa Dopamine beta -hydroxylase (D beta H) in Western blots. |
| 使用方法 | Western Blot: 1:1000 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1621 (Human); 13166 (Mouse); 25699 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Early failure of N-methyl-D-aspartate receptors and deficient spine formation induced by reduction of regulatory heme in neurons. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Dopamine beta-Hydroxylase |
| 生物活性 | Human, Primate |
| 标记 | Unconjugated |
| 背景 | Dopamine beta -hydroxylase (D beta H; also dopamine beta -monooxygenase) is a 73-77 kDa member of the copper type II ascorbate-dependent monooxygenase family. It is both soluble (73 kDa) and membrane-bound (77 kDa) (anchored by an uncleaved signal sequence), and via hydroxylation, converts dopamine into norepinepherine. Human D beta H is a copper-containing disulfide-linked homodimer that is found in neurons and adrenal medullary cells. It is 603 amino acids (aa) in length and contains a 25 aa signal sequence followed by three domains. The first is an N-terminal 120 aa DOMON domain (dopamine beta -monooxygenase N-terminal) that may either bind D beta H to the cell membrane, or participate in tetramerization. This is followed by two 150 aa Cu+-type II ascorbate-dependent monooxygenase domains (aa 182-330 and 352-512). D beta H may be most active as a dimeric-dimer/tetramer, whose association status is dependent on local Cl-concentrations. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Dopamine beta ‑Hydroxylase (N-Terminus). Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa D beta H protein. |
