Human/Mouse/Rat GluR2/3 Affinity Purified Polyclonal Ab (100 UL)
货号：
PPS051 基本售价：
5373.6 元 规格：
-
产品信息
概述| 货号 | PPS051 |
| 别名 | AMPA 2; glutamate receptor, ionotropic, AMPA 2 |
全称 | Glutamate Receptor 2/3 | | 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1:1000 dilution)
|
| 目标/特异性 | This antibody is specific for the 100 kDa GluR2/3 in Western blots of rat brain lysates. |
| 使用方法 | Western Blot: 1:1000 dilution
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | GluR2/3 |
| 生物活性 | Human, Mouse, Rat, Chicken, Zebrafish |
| 标记 | Unconjugated |
| 背景 | Rat GluR2 is a 100 kDa, 862 amino acid, 4-transmembrane (TM) glycoprotein that belongs to the ionotrophic/glutamate-gated ion channel family. Due to an impenetrate TM region 2, the N-terminus is extracellular and C-terminus intracellular. GluR2 is the most widely expressed of four AMPA receptor subunits, and contributes to the formation of a functional, presumably heterotetrameric glutamate receptor. AMPA receptors mediate fast excitatory transmission on postsynaptic membranes. The GluR2 subunit in particular regulates Ca, Zn and Na entry into the cell. It is unique among AMPA subunits in that it contains an Arginine in the second transmembrane segment (amino acid 607). This accounts for its ability to regulate ion flow. Upon neuronal insult, the Arginine can be converted to Glutamine, which allows for rapid Ca++ influx and the initiation of apoptosis. Alternatively, GluR2 levels may drop, allowing for multiple GluR combinations that allow rapid Ca++ influx. GluR2 is regulated both by palmitoylation and phosphorylation. Palmitoylation at C610 results in decreased surface expression. Phosphorylation by PKC on S880, and by Src on Y876, induces internalization. Human and rat GluR2 are 99% aa identical. Rat GluR3 is 866 aa in length and shares 72% overall aa identity with GluR2, with 85% aa identity over the C-terminal 15 amino acids. It is apparently not phosphorylated. GluR3 has a Ca++-permissive Glutamine at amino acid 612, the homolog to GluR2 R607. GluR3 is restricted in its expression pattern and is found on cerebellar Purkinje and Golgi Type II cells, neurons of the lateral amygdala, and cells of the basal ganglia. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Keinanen, K. et al. (1990) Science 249:556.
- Dong, H. et al. (1997) Nature 386:279.
- Wyszynski, M. et al. (1999) J. Neurosci. 19:6528.
- Boulter, J. et al. (1990) Science 249:1033.
- Wang, J.Q. et al. (2005) Mol. Neurobiol. 32:327.
- Soundarapandian, M.M. et al. (2005) Mol. Neurobiol. 32:145.
|
参考图片Detection of GluR2/3 by Western Blot. Western blot of rat hippocampal lysate showing the specific immunolabeling of the approximately 100 kDa GluR2/3 protein. |
