Human/Mouse/Rat/Canine/Primate APP Affinity Purified PAb (100 UL)
货号：
PPS044 基本售价：
5373.6 元 规格：
-
产品信息
概述| 货号 | PPS044 |
| 别名 | amyloid beta (A4) precursor protein-binding, family B, member 2; amyloid beta A4 precursor protein-binding family B member 2; Amyloid beta precursor protein; Amyloid beta; APP; Protease Nexin II |
全称 | Amyloid Precursor Protein | | 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1:1000 dilution)
|
| 目标/特异性 | This antibody is specific for the ~120 kDa APP in Western blots of rat brain. |
| 使用方法 | Western Blot: 1:1000 dilution
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 351 (Human); 11820 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | APP |
| 生物活性 | Human, Mouse, Rat, Canine, Chicken, Primate |
| 标记 | Unconjugated |
| 背景 | APP, or amyloid precursor protein, is a heparin-binding, 120 kDa type I transmembrane glycoprotein that belongs to the APP family of proteins. It is highly conserved across species, showing at least 97% amino acid (aa) identity between human, monkey, rat, mouse and dog. There are almost a dozen potential alternate splice forms for human APP. The neuronal (or standard) form is referred to as APP695. The 695 includes a 17 aa signal peptide and 678 aa mature region. This form contains a multidomain, 610 aa extracellular region (ECD), a 24 aa transmembrane segment (aa 625 - 648), and a short 47 aa cytoplasmic tail. Longer forms of APP, APP751 and APP770 are characterized by the presence of a 57 aa KPI, or Kunitz protease inhibitor domain in the ECD. This has protease-inhibition activity and may be involved in memory. Membrane-bound APP695 undergoes two-stage proteolytic processing. There is an initial cleavage by either an alpha - or beta -secretase, followed by cleavage by a gamma -secretase. The choice of alpha - or beta -secretase may depend upon the APP glycosylation pattern or phosphorylation events. alpha -Secretase cleavage occurs between K612 and L613. This releases a soluble APPa piece that shows proliferative activity with EGF family members on neural progenitor cells and keratinocytes. beta -secretase cleaves between M596 and D597. When followed by gamma -secretase cleavage following position V637, or A639, small 40 or 42 aa A beta peptides are released that may contribute to the formation of amyloid plaques. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Suzuki, T. et al. (2006) J. Biochem. 139:949.
- Chen, Y. and B.L. Tang (2006) Biochem. Biophys. Res. Commun. 341:1.
- Reinhard, C. et al. (2005) EMBO J. 24:3996.
- Mendendez-Gonzales, M. et al. (2005) Neurodegenerative Dis. 2:227.
- Nakagawa, K. et al. (2006) J. Neurochem. 96:924.
- Kang, J. et al. (1987) Nature 325:733.
|
参考图片Detection of Amyloid Precursor Protein by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 120 kDa APP protein. |
