概述| 货号 | PPS028 |
| 别名 | GABA subunit A receptor alpha 6; GABA(A) receptor subunit alpha-6; GABRA6; gamma-aminobutyric acid (GABA) A receptor, alpha 6; gamma-aminobutyric acid receptor subunit alpha-6; MGC116903; MGC116904 |
全称 | gamma-Aminobutyric Acid Type A Receptor, alpha-6 Polypeptide | | 应用 | Western Blot(1:1000 dilution)
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| 目标/特异性 | Human, mouse, rat, and bovine ~57 kDa GABAA R alpha 6 subunit, C-Terminus |
| 使用方法 | Western Blot: 1:1000 dilution
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2559 (Human); 14399 (Mouse); 29708 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Fusion protein from the cytosolic loop of the rat GABAA R alpha 6 subunit, C-Terminus |
| 生物活性 | Human, Mouse, Rat, Bovine |
| 标记 | Unconjugated |
| 背景 | GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha 6 isoform is a 57 kDa, 434 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 14 - 221). The expression of the subunit is strongly associated with cerebellar granule cells. The alpha 6 subunit has also been linked to alcohol sensitivity, possible due to a point mutation substitution of arginine for a glutamine at amino acid position 81 in the extracellular N-terminus. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
- Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
- Korpi, E.R. et al. (1993) Nature 361:356.
- Luddens, H. et al. (1990) Nature 346:648.
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参考图片Detection of GABAA R alpha 6 C-Terminus by Western Blot. Western blot of forebrain lysates from wild type and alpha 6 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAA R, alpha 6 subunit, C-Terminus diluted 1:1000. The antibody labeled the ~57 kDa alpha 6 subunit of the GABAA R in the wild type (control) but not in the alpha 6 knockout animals. |
