概述| 货号 | PPS027 |
| 别名 | GABA(A) receptor subunit alpha-5; GABRA5; gamma-aminobutyric acid (GABA) A receptor, alpha 5; gamma-aminobutyric acid receptor subunit alpha-5; MGC138184 |
全称 | gamma-Aminobutyric Acid Type A Receptor, alpha-5 Polypeptide | | 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1:1000 dilution)
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| 目标/特异性 | Mouse and rat ~55 kDa GABAA R alpha 5 subunit, C-Terminus |
| 使用方法 | Western Blot: 1:1000 dilution
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2558 (Human); 110886 (Mouse); 29707 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Fusion protein from the cytosolic loop of the rat GABAA R alpha 5 subunit, C-Terminus |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 背景 | GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha 5 isoform is a 439 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 25 - 232). The alpha 5 subunit is unusual in that it contains an N-terminal histidine which renders it very sensitive to zinc. It is also associated with extrasynaptic sites and seems to mediate tonic inhibition in hippocampal neurons. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
- Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
- Caraiscos, V.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:3662.
- Fisher, J.L. (2002) Neuropharmacology 42:922.
- Loebrich, S. et al. (2006) EMBO J. 25:987.
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参考图片Detection of GABAA R alpha 5 C-Terminus by Western Blot. Western blot of 7 μg of rat hippocampus showing specific immunolabeling of the ~55 kDa alpha 5 subunit of the GABAAR. |
