概述| 货号 | PPS022 |
| 别名 | ECA4; EJM; EJM5GABA(A) receptor subunit alpha-1; GABRA1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid receptor subunit alpha-1 |
全称 | gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide | | 应用 | Western Blot(1:1000 dilution)
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| 目标/特异性 | Human, mouse, rat and bovine ~51 kDa GABAA R alpha 1 subunit, C-Terminus |
| 使用方法 | Western Blot: 1:1000 dilution
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2554 (Human); 14394 (Mouse); 29705 (Rat); 282235 (Bovine) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Grp94 Protein Delivers ?-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation
Authors: XJ Di, YJ Wang, DY Han, YL Fu, AS Duerfeldt, BS Blagg, TW Mu
J. Biol. Chem., 2016;291(18):9526-39.
Species: Human
Sample Type: Recombinant Protein
Application: WB |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Fusion protein from the cytoplasmic loop GABAA R alpha 1 subunit |
| 生物活性 | Human, Mouse, Rat, Bovine, Canine, Primate, Zebrafish |
| 标记 | Unconjugated |
| 背景 | GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta, and gamma subunits. The rat alpha 1 isoform is a 50 - 52 kDa, 428 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is used as a regulatory mechanism. CaM kinase II is known to phosphorylate the alpha 1 subunit and regulate benzodiazepine binding. alpha 1 subunits are particularly abundant in the cerebellum and may contribute to GABA receptor distribution. In the hippocampus and amygdala, the alpha 1 subunit may contribute to amnesia. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
- Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
- Churn, S.B. et al. (2002) J. Neurochem. 82:1065.
- Kralic, J.E. et al. (2006) J. Comp. Neurol. 495:408.
- Sonner, J.M. et al. (2006) Mol. Pharmacol. 68:61.
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参考图片Detection of GABAA R alpha 1 C-Terminus by Western Blot Western blot of forebrain lysates from wildtype and alpha 1 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAA R, alpha 1 subunit, C-Terminus diluted 1:1000. The antibody labelled the ~51kDa alpha 1 subunit of the GABAA R (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals. |
