| 货号 | PPS035 |
| 别名 | SYN1; SYN1a; SYN1b; Synapsin 1; synapsin IBrain protein 4.1; synapsin-1; SYNI |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1:1000 dilution) Immunohistochemistry(1:2000 dilution) Immunoprecipitation(0.5 µg/100 µg cell lysate) Immunofluorescence(1:2000 dilution) |
| 目标/特异性 | This antibody is specific for the ~78 kDaSynapsin I doublet in Western blots of rat brain lysates.Immunolabeling is blocked by preadsorption of the antibody with the nativeprotein immunogen. |
| 使用方法 | Western Blot: 1:1000 dilution Immunohistochemistry: 1:2000 dilution Immunoprecipitation: 0.5 µg/100 µg cell lysate Immunofluorescence: 1:2000 dilution |
| 来源 | Thisantibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4,2.7 mM KCl, 1.5 mM KH2PO4) before use. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6853 (Human); 20964 (Mouse); 24949 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Synapsin I |
| 生物活性 | Human, Mouse, Rat, Bovine |
| 标记 | Unconjugated |
| 溶解方法 | Thisantibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4,2.7 mM KCl, 1.5 mM KH2PO4) before use. |
| 背景 | SynapsinI is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interactswith synaptic vesicles and regulates neurotransmitter release. It isconcentrated in pre‑synaptic terminals where it anchors synaptic vesicles tothe underlying cytoskeleton. Phosphorylation at various sites reduces SynapsinI binding to neurosecretory vesicles, making them available forneurotransmitter release. Rat Synapsin I is 704 amino acids (aa) in length andcontains five domains, named A-E/F. Domains A‑D lie between aminoacids 1-655, while E and F are alternate splice forms. Domain A (28 aa)regulates neurotransmitter release in a phosphorylation-dependent manner. DomainsC (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84aa) and D (235 aa) promote actin and secretory vesicle interaction, and actinnucleation. Phosphorylation of S603 and S566 in domain D, and S9 in domain A,reduces the affinity of Synapsin I for actin and secretory vesicles. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Synapsin I by Western Blot. Western blot of 10 μg of rat hippocampus lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet. |
