Human/Mouse/Rat GRIN2B/NMDAR2B Affinity Purified PAb (10 UG)【科瑞奥博】

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Human/Mouse/Rat GRIN2B/NMDAR2B Affinity Purified PAb (10 UG)

货号： PPS013 基本售价： 5373.6 元规格： -

产品信息

概述
货号	PPS013
别名	NMDA R, NR2B Subunit; NMDAR2B; NR2B
全称	Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B
反应种属	Human/Mouse/Rat
应用	Western Blot(1:1000 dilution) Immunohistochemistry(1:1000 - 1:2000 dilution)
目标/特异性	Human, mouse, rat ~180 kDa NR2B-subunit of the NMDA Receptor
使用方法	Western Blot: 1:1000 dilution Immunohistochemistry: 1:1000 - 1:2000 dilution Immunoprecipitation: 3 μL/200 μg lysate
来源	Polyclonal Rabbit IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2904 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring. Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W Mol Brain, 2012;5(0):22. Species: Rat Sample Type: Tissue Homogenates Application: WB Reduced retinal function in amyloid precursor protein-over-expressing transgenic mice via attenuating glutamate-N-methyl-d-aspartate receptor signaling. Authors: Shimazawa M, Inokuchi Y, Okuno T, Nakajima Y, Sakaguchi G, Kato A, Oku H, Sugiyama T, Kudo T, Ikeda T, Takeda M, Hara H J. Neurochem., 2008;107(1):279-90. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Fusion protein from the C-terminus of the NR2B-subunit of rat NMDA Receptor
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
背景	Rat NMDA receptor subunit 2B (NR2B) is a 1456 amino acid, 180 kDa, 3-transmembrane member of the glutamate-gated ion channel family. NMDA receptors are heteromultimers of at least two NR1 subunits and two NR2 or two (presumably) NR3 subunits. Upon ligand binding, NMDA receptors open and allow cation transit. The NR1 subunits bind glycine and are essential for the physical formation of the ion channel. NR2 subunits apparently bind glutamate and impart pharmacological properties to the receptor complex. Fully functional receptors require the presence of both glycine and glutamate. The NR1-NR2B complex has the highest affinity for glutamate. Both NR1 and NR2B subunits can be phosphorylated by protein kinase C (PKC). On NR1, PKC phosphorylates on serine at 890; on NR2B, PKC phosphorylates on tyrosine at 1472. Phosphorylation is considered to promote ion transit and impact NR2B interaction with downstream signaling molecules.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Grosshans, D.R. and M.D. Browning (2001) J. Neurochem. 76:737. Cheung, H. et al. (2003) J. Neurochem. 86:1441. Gibb, A.J. (2004) Trends Neurosci. 27:7. Liu, Y. and J. Zhang (2000) Chin. Med. J. 113:948.

参考图片

Detection of GRIN2B/NMDAR2B by Western Blot Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA Receptor. The labeling by the antibody was blocked by the C-terminal fusion protein of the NR2B subunit of the NMDA Receptor used as immunogen (not shown).