Human/Mouse/Rat GRIN2A/NMDAR2A Affinity Purified PAb (10 UG)【科瑞奥博】

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Human/Mouse/Rat GRIN2A/NMDAR2A Affinity Purified PAb (10 UG)

货号： PPS012 基本售价： 5373.6 元规格： -

产品信息

概述
货号	PPS012
别名	EPND; FESD; GluN2A; LKS; NMDA R, NR2A Subunit; NMDAR2A; NR2A
全称	Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2A
反应种属	Human/Mouse/Rat
应用	Western Blot(1:1000 dilution) Immunohistochemistry(1:1000 to 1:2000 dilution)
目标/特异性	Specific for the ~180 kDa NR2A subunit of the NMDA Receptor in Western blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
使用方法	Western Blot: 1:1000 dilution Immunohistochemistry: 1:1000 to 1:2000 dilution Immunoprecipitation: 3 μL per 200 μg lysate
来源	Polyclonal Rabbit IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2903 (Human); 14811 (Mouse); 24409 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lrp4 in astrocytes modulates glutamatergic transmission Authors: Xiang-Dong Sun Nat Neurosci, 2016;0(0):. Species: Mouse Sample Type: Tissue Homogenates Application: WB Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring. Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W Mol Brain, 2012;5(0):22. Species: Rat Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Fusion protein from the C-terminus of the NR2A subunit of rat NMDA Receptor
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
背景	NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg⁺⁺ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes, which code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitutes the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Glutamate Receptors Interactive Pathway: Synaptic Neurotransmission: Glutamatergic Excitation Neurodegenerative Disease Neurotransmitter Receptors, Transporters, and Ion Channels Synaptic EphB2-NMDA R Dynamics: A Target for Nervous System Disease

参考图片

Detection of GRIN2A/NMDAR2A by Western Blot. Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA Receptor. The labeling by the antibody was blocked by the N-terminal NR2A-subunit of the NMDA Receptor fusion protein (not shown).