| 货号 | AF3864 |
| 别名 | B2M; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin |
| 反应种属 | Rat |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects rat beta 2-Microglobulin ( beta 2M) in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 567 (Human); 12010 (Mouse); 24223 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat beta 2-Microglobulin Ile21-Met119 Accession # P07151 |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | beta2-Microglobulin ( beta 2M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta 2M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta 2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat beta 2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of beta 2‑Microglobulin in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Rat beta 2‑Microglobulin Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3864, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). |
