| 货号 | AF2466 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：AF2466；Detection antibody：BAF2466; and protein: 2466-RA-050 |
| 别名 | DIRA; ICIL-1RA; IL1F3; IL-1F3; IL1RA; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 | 全称 | Interleukin 1 Receptor Antagonist |
| 反应种属 | Equine |
| 应用 | Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects equine IL‑1ra/IL‑1F3 in ELISAs and Western blots. In sandwich immunoassays, less than 20% cross-reactivity with recombinant mouse IL-1ra is observed, less than 5% cross-reactivity with recombinant rat IL-1ra is observed, less than 2% cross-reactivity with recombinant porcine IL-1ra is observed, less than 0.4% cross-reactivity with recombinant human IL-1ra is observed, and less than 0.2% cross-reactivity with recombinant equine IL-1beta is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant equine IL‑1ra/IL‑1F3 His26-Gln177 Accession # O18999 |
| 生物活性 | Equine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Secreted equine IL-1 receptor antagonist (IL-1ra) is a presumably 22‑25 kDa glycoprotein produced by variety of cell types that antagonizes IL-1 activity (1‑3). It is a member of the IL-1 family of proteins that includes IL-1 alpha and IL-1 beta. Although there is little amino acid (aa) identity (<30%) among the three IL-1 family members, all molecules bind to the same receptors, all show a beta -trefoil structure, and all are believed to have evolved from a common ancestral gene (1‑4). Equine IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence plus a 152 aa mature region. There is one intrachain disulfide bond and one potential N-linked glycosylation site (3, 5, 6). Mature equine sIL-1ra is 78%, 78%, 80%, 82%, and 76% aa identical to mature mouse, human, porcine, canine and bovine IL‑1ra, respectively. In human, three non-secreted IL-1ra isoforms have also been identified. It is unknown if such an analogous situation exists in equine. Cells known to secrete IL-1ra include fibroblasts, vascular smooth muscle cells, intestinal columnar epithelium, chondrocytes, macrophages, mast cells, neutrophils and hepatocytes. There are two type I transmembrane glycoprotein receptors for IL-1ra. The first is the bioactive 80 kDa type I IL-1 receptor (IL-1 RI), and the second is the inert (decoy) 65 kDa type II IL-1 receptor. IL-1ra binding to IL-1 RI competitively blocks IL-1 ( alpha or beta ) binding to the same receptor. This results in receptor ligation without activation (1, 7). The type II IL-1 receptor is inert, and any binding of IL-1ra not only fails to block co-existing IL-1 activity, but may actually potentiate it by removing an IL-1 antagonist. Functionally, all activities attributed to IL-1ra are explained by its role as a competitive inhibitor of IL-1 binding to IL-1 RI (1, 2, 8, 9). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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IL‑1ra/IL‑1F3 in Equine PBMCs. IL‑1ra/IL‑1F3 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2466) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
