Mouse IL-13 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Mouse IL-13 Affinity Purified Polyclonal Ab (100 UG)

货号： AF-413-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-413-NA
别名	ALRHMGC116789; BHR1interleukin-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
全称	Interleukin 13
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse IL-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat IL‑13 is observed, and approximately 10% cross-reactivity with recombinant human IL‑13 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.05-0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema. Authors: Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M PLoS ONE, 2015;10(3):e0119797. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Small interfering RNA against transcription factor STAT6 inhibits allergic airway inflammation and hyperreactivity in mice. Authors: Darcan-Nicolaisen Y, Meinicke H, Fels G, Hegend O, Haberland A, Kuhl A, Loddenkemper C, Witzenrath M, Kube S, Henke W, Hamelmann E J. Immunol., 2009;182(12):7501-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo. Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK J. Allergy Clin. Immunol., 2008;122(3):625-32. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development. Authors: Khaled WT, Read EK, Nicholson SE, Baxter FO, Brennan AJ, Came PJ, Sprigg N, McKenzie AN, Watson CJ Development, 2007;134(15):2739-50. Species: Mouse Sample Type: Whole Cells Application: ICC Modulation of airway remodeling and airway inflammation by peroxisome proliferator-activated receptor gamma in a murine model of toluene diisocyanate-induced asthma. Authors: Lee KS, Park SJ, Kim SR, Min KH, Jin SM, Lee HK, Lee YC J. Immunol., 2006;177(8):5248-57. Species: Mouse Sample Type: BALF Application: WB Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling. Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK J. Immunol., 2006;176(12):7495-501. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL-13 Ser26-Phe131 Accession # P20109
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Wills-Karp, M. (2004) Immunol. Rev. 202:175. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265. Brown, K.D. et al. (1989) J. Immunol. 142:679. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231. Ruetten, H. and C. Thiemermann (1997) Shock 8:409. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464. Tabata, Y. et al. (2007) J. Immunol. 177:7905. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

参考图片

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL‑13 (Catalog # 413-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND₅₀ is typically 0.05‑0.15 µg/mL.

IL‑13 in Mouse Splenocytes. IL‑13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.