Mouse TNF-alpha Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Mouse TNF-alpha Affinity Purified Polyclonal Ab (100 UG)

货号： AF-410-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-410-NA
描述	For ELISA the Antibody Pairs information：Capture antibody：AF-410-NA；Detection antibody：BAF410; and protein: 410-MT-010
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Mouse
应用	Western Blot,CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF‑ alpha is observed and less than 1% cross-reactivity with recombinant human TNF-alpha is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse TNF‑ alpha and 1 µg/mL actinomycin D.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells Authors: SY Lim, AE Yuzhalin, AN Gordon-Wee, RJ Muschel Oncogene, 2016;0(0):. Species: Mouse Sample Type: cell culture supernate Application: Neut Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Nat Med, 2014;20(4):377-84. Species: Mouse Sample Type: Whole Cells Application: Bioassay FcgammaRIIb inhibits immune complex-induced VEGF-A production and intranodal lymphangiogenesis. Authors: Clatworthy M, Harford S, Mathews R, Smith K Proc Natl Acad Sci U S A, 2014;111(50):17971-6. Species: Mouse Sample Type: Whole Cells Application: Neut Cytokine conditioning enhances systemic delivery and therapy of an oncolytic virus. Authors: Ilett E, Kottke T, Donnelly O, Thompson J, Willmon C, Diaz R, Zaidi S, Coffey M, Selby P, Harrington K, Pandha H, Melcher A, Vile R Mol Ther, 2014;22(10):1851-63. Species: Mouse Sample Type: Whole Cells Application: Neut Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways. Authors: Li B, Shi Y, Shu J, Gao J, Wu P, Tang S J Biol Chem, 2013;288(19):13610-9. Species: Mouse Sample Type: Tissue Homogenates Application: WB Mice lacking C1q are protected from high fat diet-induced hepatic insulin resistance and impaired glucose homeostasis. Authors: Hillian, Antoinet, McMullen, Megan R, Sebastian, Becky M, Roychowdhury, Sanjoy, Kashyap, Sangeeta, Schauer, Philip R, Kirwan, John P, Feldstein, Ariel E, Nagy, Laura E J Biol Chem, 2013;288(31):22565-75. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease. Authors: Kim A, Feng P, Ohkuri T, Sauers D, Cohn ZJ, Chai J, Nelson T, Bachmanov AA, Huang L, Wang H PLoS ONE, 2012;7(4):e35588. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells. Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS J. Clin. Invest., 2011;121(10):3846-59. Species: Mouse Sample Type: In Vivo Application: In vivo TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM J. Clin. Invest., 2009;119(10):2954-64. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model. Authors: Wendt W, Schulten R, Stichel CC, Lubbert H J. Neurochem., 2009;110(6):1931-41. Species: Mouse Sample Type: Cell Lysates Application: WB Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9. Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M J. Neurochem., 2009;111(6):1409-24. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T Microbes Infect., 2009;11(4):524-30. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Cooperative phagocytes: resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions. Authors: Joly S, Francke M, Ulbricht E, Beck S, Seeliger M, Hirrlinger P, Hirrlinger J, Lang KS, Zinkernagel M, Odermatt B, Samardzija M, Reichenbach A, Grimm C, Reme CE Am. J. Pathol., 2009;174(6):2310-23. Species: Mouse Sample Type: Whole Tissue Application: IHC Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA J. Biol. Chem., 2009;0(0):. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T Immunology, 2008;125(2):272-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Rod-Shaped monocytes patrol the brain vasculature and give rise to perivascular macrophages under the influence of proinflammatory cytokines and angiopoietin-2. Authors: Audoy-Remus J, Richard JF, Soulet D, Zhou H, Kubes P, Vallieres L J. Neurosci., 2008;28(41):10187-99. Species: Mouse Sample Type: Whole Cells Application: Neut CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation. Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM Biochem. Pharmacol., 2008;76(1):79-90. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. Authors: Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK J. Immunol., 2008;180(4):2553-61. Species: Mouse Sample Type: Serum Application: ELISA Development Tumor-specific Th17-polarized cells eradicate large established melanoma. Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Blood, 2008;112(2):362-73. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Hypoxia enhances lysosomal TNF-alpha degradation in mouse peritoneal macrophages. Authors: Lahat N, Rahat MA, Kinarty A, Weiss-Cerem L, Pinchevski S, Bitterman H Am. J. Physiol., Cell Physiol., 2008;295(1):C2-12. Species: Mouse Sample Type: Whole Cells Application: Flow Antipsoriatic effects of avarol-3-thiosalicylate are mediated by inhibition of TNF-alpha generation and NF-kappaB activation in mouse skin. Authors: Amigo M, Paya M, De Rosa S, Terencio MC Br. J. Pharmacol., 2007;152(3):353-65. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor. Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ Eur. J. Pharmacol., 2007;573(1):221-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury. Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA J. Immunol., 2006;177(1):612-20. Species: Mouse Sample Type: BALF Application: ELISA Development Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D. Authors: Murata T, Tsuboi M, Hikita K, Kaneda N J. Biol. Chem., 2006;281(32):22503-16. Species: Mouse Sample Type: Whole Cells Application: Neut Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK Infect. Immun., 2006;74(12):6730-8. Species: Mouse Sample Type: BALF Application: ELISA Development Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG J. Immunol., 2006;177(3):1967-74. Species: Mouse Sample Type: Plasma Application: ELISA Development T lineage differentiation from human embryonic stem cells. Authors: Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11742-7. Species: Mouse Sample Type: Whole Cells Application: ICC Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1. Authors: Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11784-9. Species: Mouse Sample Type: Whole Cells Application: ICC Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice. Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG Inflamm. Res., 2006;55(11):457-64. Species: Mouse Sample Type: Synovial Fluid Application: ELISA Development Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH J Ethnopharmacol, 2006;106(3):418-24. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells. Authors: Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M Mol. Cell. Neurosci., 2006;31(1):149-60. Species: Mouse Sample Type: Whole Cells Application: Neut Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis. Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A J. Immunol., 2006;177(12):8650-7. Species: Mouse Sample Type: Peritoneal Lavage Application: ELISA Development Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4). Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ Eur. J. Immunol., 2006;36(8):2025-34. Species: Mouse Sample Type: Peritoneal Exudate Application: ELISA Development Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons. Authors: Gonzalez de Aguilar JL, Dupuis L, Larmet Y Neurobiol. Dis., 2005;19(1):129-41. Species: Mouse Sample Type: Cell Lysates Application: WB Tumor necrosis factor-like weak inducer of apoptosis increases the permeability of the neurovascular unit through nuclear factor-kappa B pathway activation. Authors: Polavarapu R, Gongora MC, Winkles JA, Yepes M J. Neurosci., 2005;25(44):10094-100. Species: Mouse Sample Type: Application: In vivo The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors. Authors: Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS J. Invest. Dermatol., 2005;125(5):1053-62. Species: Mouse Sample Type: Tissue Homogenates Application: WB Transcriptional profiling of lipopolysaccharide-induced acute lung injury. Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS Infect. Immun., 2004;72(12):7247-56. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice. Authors: Tezel G, Yang X, Yang J, Wax MB Brain Res., 2004;996(2):202-12. Species: Rat Sample Type: Whole Tissue Application: IHC Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity. Authors: LaBue M, Colburn KK, Green LM Thyroid, 2004;14(4):249-62. Species: Mouse Sample Type: Cell Culture Supernates Application: IP Selective macrophage suppression during sepsis. Authors: Ellaban E, Bolgos G, Remick D Cell. Immunol., 2004;231(1):103-11. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Elevated levels of tumor necrosis factor alpha (TNF-alpha) in human immunodeficiency virus type 1-transgenic mice: prevention of death by antibody to TNF-alpha. Authors: De SK, Devadas K, Notkins AL J. Virol., 2002;76(22):11710-4. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) Leu80-Leu235 Accession # P06804
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Tumor necrosis factor alpha (TNF-alpha, also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types. Mouse TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70% - 77% with bovine, canine, cotton rat, equine, feline, human, porcine, rat, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha. TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-alpha regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-alpha is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adipocytokines Cancer Biomarkers Conventional/Classical Dendritic Cells Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy EMT Induction gamma delta T Cells ICC / IHC Images: TNF-alpha Inflammatory Mediators Inflammatory/Monocyte-derived Dendritic Cells Interactive Pathway: Adipocytokines & Insulin Signaling Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Natural Killer T (NKT) Cells Neuroinflammation Obesity Plasmacytoid Dendritic Cells Preeclampsia Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members T Cell Cytokine Signaling Technical Notes: R&D Systems Is With You Every Step of the Way - Analyzing Proteins of Interest Th1 Cells Th22 Cells TNF Superfamily Ligands

参考图片

TNF‑ alpha in Mouse T Cells. TNF‑ alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑410‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Mouse TNF‑ alpha Antibody. Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND₅₀ is typically 0.1-0.4 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNF‑ alpha in RAW 264.7 Mouse Cell Line. TNF‑ alpha was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.