Mouse IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Mouse IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (100 UG)

货号： AF-401-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-401-NA
别名	catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Mouse
应用	Western Blot(0.25 µg/mL) Simple Western(2.5 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat IL‑1 beta is observed and less than 10% cross-reactivity with recombinant porcine IL-1 beta and recombinant human IL-1 beta is observed.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 2.5 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS Authors: Yong Hwan Park Nat Immunol, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Lymphatic vessels regulate immune microenvironments in human and murine melanoma J Clin Invest, 2016;0(0):. Species: Human Sample Type: Tissue Homogenates Application: WB Identification of QS-21 as an Inflammasome-activating Molecular Component of Saponin Adjuvants. Authors: Marty-Roix R, Vladimer G, Pouliot K, Weng D, Buglione-Corbett R, West K, Macmicking J, Chee J, Wang S, Lu S, Lien E J Biol Chem, 2016;291(3):1123-36. Species: Mouse Sample Type: Cell Lysates Application: WB Enzymatically inactive procaspase-1 stabilizes the ASC-pyroptosome and supports pyroptosome spreading during cell division J Biol Chem, 2016;0(0):. Species: Mouse Sample Type: Cell Culture Supernates Application: Bead-based Immunoassay CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells J Leukoc Biol, 2016;0(0):. Species: AF-401-na Sample Type: Whole Cells Application: blocking/neutralization C13orf31 (FAMIN) is a central regulator of immunometabolic function Nat Immunol, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation Authors: X Wang, DK Shaw, OS Sakhon, GA Snyder, EJ Sundberg, L Santambrog, FS Sutterwala, JS Dumler, KA Shirey, DJ Perkins, K Richard, AC Chagas, E Calvo, J Kopecký, M Kotsyfakis, JH Pedra Infect Immun, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB HIF-1? Is an Essential Mediator of IFN-?-Dependent Immunity to Mycobacterium tuberculosis J Immunol, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes. Authors: Maier N, Leppla S, Moayeri M J Immunol, 2015;194(6):2776-85. Species: Mouse Sample Type: Cell Lysates Application: WB Small heterodimer partner interacts with NLRP3 and negatively regulates activation of the NLRP3 inflammasome. Authors: Yang C, Kim J, Kim T, Lee P, Kim S, Lee H, Shin D, Nguyen L, Lee M, Jin H, Kim K, Lee C, Kim M, Park S, Kim J, Choi H, Jo E Nat Commun, 2015;6(0):6115. Species: Mouse Sample Type: Cell Lysates Application: WB Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules. Authors: Sadeghi H, Lockmann A, Hund A, Samavedam U, Pipi E, Vafia K, Hauenschild E, Kalies K, Pas H, Jonkman M, Iwata H, Recke A, Schon M, Zillikens D, Schmidt E, Ludwig R J Immunol, 2015;194(8):3656-63. Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida. Authors: Meunier E, Wallet P, Dreier R, Costanzo S, Anton L, Ruhl S, Dussurgey S, Dick M, Kistner A, Rigard M, Degrandi D, Pfeffer K, Yamamoto M, Henry T, Broz P , 2015;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice. Authors: Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K J Immunol, 2015;195(3):1233-41. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Arsenic trioxide and other arsenical compounds inhibit the NLRP1, NLRP3, and NAIP5/NLRC4 inflammasomes. Authors: Maier N, Crown D, Liu J, Leppla S, Moayeri M J Immunol, 2014;192(2):763-70. Species: Human Sample Type: Cell Lysates Application: WB Endothelial NLRP3 inflammasome activation and enhanced neointima formation in mice by adipokine visfatin. Authors: Xia M, Boini K, Abais J, Xu M, Zhang Y, Li P Am J Pathol, 2014;184(5):1617-28. Species: Mouse Sample Type: Whole Tissue Application: IHC Activation of NLRP3 inflammasome by crystalline structures via cell surface contact. Authors: Hari A, Zhang Y, Tu Z, Detampel P, Stenner M, Ganguly A, Shi Y Sci Rep, 2014;4(0):7281. Species: Mouse Sample Type: Cell Lysates Application: WB Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways. Authors: Li B, Shi Y, Shu J, Gao J, Wu P, Tang S J Biol Chem, 2013;288(19):13610-9. Species: Mouse Sample Type: Tissue Homogenates Application: WB Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets. Authors: El-Zaatari M, Kao J, Tessier A, Bai L, Hayes M, Fontaine C, Eaton K, Merchant J PLoS ONE, 2013;8(3):e58935. Species: Mouse Sample Type: Whole Tissue Application: IHC Fluorescence Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome. Authors: Misawa T, Takahama M, Kozaki T, Lee H, Zou J, Saitoh T, Akira S Nat Immunol, 2013;14(5):454-60. Species: Mouse Sample Type: Peritoneal Fluid Application: WB Activation of microglia induces symptoms of Parkinson's disease in wild-type, but not in IL-1 knockout mice. Authors: Tanaka S, Ishii A, Ohtaki H, Shioda S, Yoshida T, Numazawa S J Neuroinflammation, 2013;10(0):143. Species: Mouse Sample Type: tissue Application: IHC Frozen Caspase-11 increases susceptibility to Salmonella infection in the absence of caspase-1. Authors: Broz P, Ruby T, Belhocine K, Bouley D, Kayagaki N, Dixit V, Monack D Nature, 2012;490(7419):288-91. Species: Mouse Sample Type: Cell Culture Supernates Application: WB The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J Nature, 2012;492(7427):123-7. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus. Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C Infect. Immun., 2012;80(1):410-7. Species: Mouse Sample Type: Whole Cells Application: Neut IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells. Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS J. Clin. Invest., 2011;121(10):3846-59. Species: Mouse Sample Type: In Vivo Application: In vivo Coordinated Host Responses during Pyroptosis: Caspase-1-Dependent Lysosome Exocytosis and Inflammatory Cytokine Maturation. Authors: Bergsbaken T, Fink SL, den Hartigh AB, Loomis WP, Cookson BT J. Immunol., 2011;187(5):2748-54. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis. Authors: Carmi Y, Rinott G, Dotan S, Elkabets M, Rider P, Voronov E, Apte RN J. Immunol., 2011;186(6):3462-71. Species: Mouse Sample Type: In Vivo Application: In vivo Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury. Authors: Kim D, You B, Lim H, Lee SJ Mol Pain, 2011;7(0):74. Species: Mouse Sample Type: Whole Cells Application: ICC IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN J. Immunol., 2011;187(9):4835-43. Species: Mouse Sample Type: In Vivo Application: In vivo Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes. Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, ONeill LA Nat. Immunol., 2010;11(10):897-904. Species: Mouse Sample Type: Cell Lysates Application: WB Tyrosine kinase 2 controls IL-1beta production at the translational level. Authors: Radwan M, Stiefvater R, Grunert T, Sharif O, Miller I, Marchetti-Deschmann M, Allmaier G, Gemeiner M, Knapp S, Kovarik P, Muller M, Strobl B J. Immunol., 2010;185(6):3544-53. Species: Mouse Sample Type: Cell Lysates Application: WB Interleukin-1beta mediates the extra-intestinal thrombosis associated with experimental colitis. Authors: Yoshida H, Russell J, Senchenkova EY, Almeida Paula LD, Granger DN Am. J. Pathol., 2010;177(6):2774-81. Species: Mouse Sample Type: In Vivo Application: In vivo Cytotoxins of the human pathogen Aeromonas hydrophila trigger, via the NLRP3 inflammasome, caspase-1 activation in macrophages. Authors: McCoy AJ, Koizumi Y, Toma C, Higa N, Dixit V, Taniguchi S, Tschopp J, Suzuki T Eur. J. Immunol., 2010;40(10):2797-803. Species: Mouse Sample Type: Cell Lysates Application: WB Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors. Authors: Ghiringhelli F, Apetoh L, Tesniere A, Aymeric L, Ma Y, Ortiz C, Vermaelen K, Panaretakis T, Mignot G, Ullrich E, Perfettini JL, Schlemmer F, Tasdemir E, Uhl M, Genin P, Civas A, Ryffel B, Kanellopoulos J, Tschopp J, Andre F, Lidereau R, McLaughlin NM, Haynes NM, Smyth MJ, Kroemer G, Zitvogel L Nat. Med., 2009;15(10):1170-8. Species: Mouse Sample Type: Whole Cells Application: Neut Externalization of the leaderless cytokine IL-1F6 occurs in response to lipopolysaccharide/ATP activation of transduced bone marrow macrophages. Authors: Martin U, Scholler J, Gurgel J, Renshaw B, Sims JE, Gabel CA J. Immunol., 2009;183(6):4021-30. Species: Mouse Sample Type: Cell Lysates Application: WB Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice. Authors: Hyvarinen K, Tuomainen AM, Laitinen S, Bykov IL, Tormakangas L, Lindros K, KÃƒÂ¤kelÃƒÂ¤ R, Alfthan G, Salminen I, Jauhiainen M, Kovanen PT, Leinonen M, Saikku P, Pussinen PJ Infect. Immun., 2009;77(8):3442-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8. Authors: Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R J. Exp. Med., 2008;205(9):1967-73. Species: Mouse Sample Type: Cell Lysates Application: WB Japanese Encephalitis Virus infection induces IL-18 and IL-1beta in microglia and astrocytes: correlation with in vitro cytokine responsiveness of glial cells and subsequent neuronal death. Authors: Das S, Mishra MK, Ghosh J, Basu A J. Neuroimmunol., 2008;195(1):60-72. Species: Mouse Sample Type: Tissue Homogenates Application: WB Toll-like receptor 4 (TLR4)-dependent proinflammatory and immunomodulatory properties of the glycoinositolphospholipid (GIPL) from Trypanosoma cruzi. Authors: Medeiros MM, Peixoto JR, Oliveira AC, Cardilo-Reis L, Koatz VL, Van Kaer L, Previato JO, Mendonca-Previato L, Nobrega A, Bellio M J. Leukoc. Biol., 2007;82(3):488-96. Species: Mouse Sample Type: In Vivo Application: In vivo Topical acetone treatment induces neurogenic oedema on the sensitized mouse ear: an in vivo study using transient receptor potential vanilloid 1 (TRPV1) receptor knockout mice. Authors: Pozsgai G, Sandor K, Perkecz A, Szolcsanyi J, Helyes Z, Brain SD, Pinter E Inflamm. Res., 2007;56(11):459-67. Species: Mouse Sample Type: In Vivo Application: In vivo Inflammasome-mediated production of IL-1beta is required for neutrophil recruitment against Staphylococcus aureus in vivo. Authors: Miller LS, Pietras EM, Uricchio LH, Hirano K, Rao S, Lin H, OConnell RM, Iwakura Y, Cheung AL, Cheng G, Modlin RL J. Immunol., 2007;179(10):6933-42. Species: Mouse Sample Type: Tissue Homogenates Application: WB Granulocyte colony-stimulating factor impairs liver regeneration in mice through the up-regulation of interleukin-1beta. Authors: Ogiso T, Nagaki M, Takai S, Tsukada Y, Mukai T, Kimura K, Moriwaki H J. Hepatol., 2007;47(6):816-25. Species: Mouse Sample Type: In Vivo Application: In vivo The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta. Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI Genes Dev., 2006;20(18):2527-38. Species: Mouse Sample Type: Whole Cells Application: Neut Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration. Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S Brain, 2005;128(0):854-66. Species: Mouse Sample Type: Application: In vivo Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum. Authors: Banks WA, Farr SA, La Scola ME J. Pharmacol. Exp. Ther., 2001;299(2):536-41. Species: Mouse Sample Type: In Vivo Application: In vivo Biglycan, a danger signal that activates the NLRP3 inflammasome via toll-like and P2X receptors. Authors: Babelova A, Moreth K, Tsalastra-Greul W, Zeng-Brouwers J, Eickelberg O, Young MF, Bruckner P, Pfeilschifter J, Schaefer RM, Schaefer R, Grone HJ, Schaefer L J Biol Chem, 0;284(36):24035-48. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL-1 beta /IL-1F2 Val118-Ser269 Accession # NP_032387
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Biomarkers Chronic IL-1 beta-induced Neuroinflammation Is it Really that Bad? Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis ICC / IHC Images: IL-1 beta/IL-1F2 IL-1 Family Infectious Diseases Inflammasomes: Intracellular Regulators of Pathogen Inflammatory Mediators Interactive Pathway: IL-1 Family Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Macrophage Activation Markers Mini-review: Interleukin 1 Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) NCR-ILC3 Neuroinflammation NOD-like Receptors and the Inflammasome Platelets Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th17 Cells Th9 Cells

参考图片

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse
IL-1 beta /IL-1F2 Antibody. Recombinant Mouse
IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA). The ND₅₀ is typically 0.05-0.25 µg/mL in the presence of concanavalin A (1.25 µg/mL).

IL‑1 beta /IL‑1F2 in Mouse Thymus. IL‑1 beta /IL‑1F2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human and Mouse IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-)ortreated (+) with 200 nM PMA for 24 hoursand 10 μg/mL LPS for 4 hours andRAW 264.7 mouse monocyte/macrophagecell line untreated (-)or treated (+) with10 μg/mL LPS for 24 hours. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse IL‑1 beta /IL‑1F2 by Simple Western^TM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

IL‑1 beta /IL‑1F2 in MCF‑7 Human Cell Line. IL‑1 beta /IL‑1F2 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.