| 货号 | AF1069 |
| 别名 | Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; resistin; RETN; RETN1; RSTNXCP1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Resistin in direct ELISAs and Western blots. In this format, less than 2% cross-reactivity with recombinant human Resistin and recombinant mouse RELM beta is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56729 (Human); 57264 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Resistin (R&D Systems, Catalog # 1069-RN) Ser21-Ser114 Accession # Q99P87 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Resistin, also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a member of a family of secreted cysteine-rich peptide hormones that also includes Resistin-like molecules RELM alpha, beta, and gamma. These molecules play important roles in inflammation, glucose metabolism, and insulin resistance. Mature mouse Resistin is a 12 kDa protein with an N-terminal alpha -helical domain and a C-terminal beta -sandwich domain that is stabilized by multiple intrachain disulfide bonds. Resistin circulates as noncovalent trimers and disulfide-linked hexamers, with the trimeric form showing greater bioactivity. Resistin can also form multimers with RELM beta. Mature mouse Resistin shares 56% and 72% amino acid (aa) sequence identity with human and rat Resistin, respectively. It shares 34% - 42% aa sequence identity with mouse RELM alpha, beta, and gamma. In rodents, Resistin is expressed by adipocytes and in the pituitary and arcuate nucleus of the hypothalamus. It is upregulated during adipogenesis, in obesity, and by insulin or a high carbohydrate diet. This is in contrast to human Resistin which is produced by macrophages and monocytes but not by adipocytes. Mouse Resistin induces proinflammatory molecule production in adipocytes and promotes hepatic gluconeogenesis and insulin resistance. Human Resistin promotes lipolysis by human and mouse adipocytes, but mouse Resistin does not promote lipolysis by adipocytes of either species. Both mouse and human Resistin promote vascular endothelial cell sprouting in vitro and inflammatory reactions in vivo. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Resistin in Mouse Brain. Resistin was detected in perfusion fixed frozen sections of mouse brain (caudate putamen) using 15 µg/mL Mouse Resistin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1069) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
