| 货号 | AF902 |
| 别名 | 72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) | 全称 | Matrix Metalloproteinase 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human MMP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50-100% cross-reactivity with recombinant mouse MMP-2 is observed, approximately 15% cross-reactivity with recombinant human (rh) MMP‑7 and less than 5% cross-reactivity with rhMMP‑1, rhMMP‑3, rhMMP‑8, rhMMP‑9, rhMMP‑10, rhMMP‑12, rhMMP‑13, rhMMP-14, rhMMP-15, rhMMP-16, rhMMP-17, rhMMP-19, and rhMMP-24 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4313 (Human); 17390 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human MMP‑2 Ile34-Cys660 Accession # P08253 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human MMP‑2 by Western Blot. Western blot shows lysate of U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP‑2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
MMP‑2 in Human Ovarian Cancer Tissue. MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
MMP‑2 in Human Ovary. MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human MMP‑2 by Simple WesternTM. Simple Western lane view shows lysates of U‑118‑MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MMP‑2 at approximately 78 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
