| 货号 | AF5736 |
| 别名 | CAV; CAV1; caveolae protein, 22kD; caveolin 1, caveolae protein, 22kDa; cell growth-inhibiting protein 32; MSTP085; VIP21 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Simple Western(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Caveolin-1 in Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL Simple Western: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 857 (Human); 12389 (Mouse); 25404 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Caveolin-1 Ser2-Ser104 Accession # Q03135 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae which are cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and the regulation of lipid rafts and signal transduction. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105 - 125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Alternate splicing in human, mouse, and rat Caveolin-1 generates an isoform with a deletion of the N-terminal 31 residues. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Caveolin‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A431 human epithelial carcinoma cell line, and A549 human lung carcinoma cell line. PVDF Membrane was probed with 0.2 µg/mL of Goat Anti-Human Caveolin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Caveolin‑1 at approximately 21 to 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Caveolin‑1 in Human Liver. Caveolin‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Caveolin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human Caveolin‑1 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Caveolin‑1 at approximately 29 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Caveolin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
