Human TRAIL/TNFSF10 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human TRAIL/TNFSF10 Affinity Purified Polyclonal Ab (100 UG)

货号： AF375 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF375
别名	Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10
全称	TNF-related Apoptosis-inducing Ligand
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human TRAIL/TNFSF10 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRAIL is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.03-0.06 µg/mL in the presence of 12 ng/mL Recombinant Human TRAIL/TNFSF10, and 1 µg/mL actinomycin D.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8743 (Human); 22035 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma. Authors: Kim, Seong Mu, Woo, Ji Sun, Jeong, Chang Hy, Ryu, Chung He, Jang, Jae-Deog, Jeun, Sin-Soo Stem Cells Transl Med, 2014;3(2):172-82. Species: Human Sample Type: Cell Lysates Application: WB KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis. Authors: Navenot JM, Fujii N, Peiper SC Mol. Pharmacol., 2009;75(5):1074-83. Species: Human Sample Type: Whole Cells Application: Neut Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway. Authors: Chamoux E, Houde N, LEriger K, Roux S J. Cell. Physiol., 2008;216(2):536-42. Species: Human Sample Type: Whole Cells Application: ICC Cloning and apoptosis-inducing activities of canine and feline TRAIL. Authors: Rong S, Cai JH, Andrews J Mol. Cancer Ther., 2008;7(7):2181-91. Species: Canine Sample Type: recombinant TRAIL Application: Immunopurification Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage. Authors: Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK J. Exp. Med., 2007;204(3):667-80. Species: Human Sample Type: Whole Cells Application: Neut TNF-related apoptosis-inducing ligand mediates human neuronal apoptosis: links to HIV-1-associated dementia. Authors: Ryan LA, Peng H, Erichsen DA, Huang Y, Persidsky Y, Zhou Y, Gendelman HE, Zheng J J. Neuroimmunol., 2004;148(1):127-39. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Apoptosis-mediating receptor-ligand systems in human retinal pigment epithelial cells. Authors: Hueber A, Aduckathil S, Kociok N, Welsandt G, Dinslage S, Kirchhof B, Esser PJ Chin. Med. J., 2002;240(7):551-6. Species: Human Sample Type: Whole Tissue Application: IHC Fresh/Frozen
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human TRAIL/TNFSF10 (R&D Systems, Catalog # 375-TL) Thr95-Gly281 Accession # P50591
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TRAIL is a type II transmembrane protein belonging to the TNFsuperfamily and is now designated TNFSF10. TRAIL is active as ahomotrimer and is produced by a variety of cell types in both themembrane bound form and also as a soluble molecule. It binds to any ofthe four TRAIL receptors as well as to Osteoprotegerin.
运输条件	Blue Ice
存放说明	-20℃
参考文献	gamma delta T Cells Human CD Marker Products ICC / IHC Images: TRAIL/TNFSF10 Isolation, Culture, and Identification of Natural Killer Cells Natural Killer T (NKT) Cells Regulation of Apoptosis by TNF Superfamily Members Regulatory T Cells (Tregs) TNF Superfamily TNF Superfamily Ligands

参考图片

Detection of Human TRAIL/TNFSF10 by Western Blot. Western blot shows lysates of 786‑O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017).Specific bands were detected for TRAIL/TNFSF10 at approximately 27 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TRAIL/TNFSF10 in Human Brain. TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Cytotoxicity Induced by TRAIL/TNFSF10 and Neutralization by Human TRAIL/TNFSF10 Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375). The ND₅₀ is typically
0.03-0.06 µg/mL.