Human CCL3/MIP-1 alpha Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human CCL3/MIP-1 alpha Affinity Purified Polyclonal Ab (100 UG)

货号： AF-270-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-270-NA
描述	For ELISA the Antibody Pairs information：Capture antibody：AF-270-NA；Detection antibody：BAF270; and protein: 270-LD-010
别名	C-C motif chemokine 3; MIP1-(a); AI323804; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3
反应种属	Human
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human CCL3/MIP‑1 alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) MIP-1 beta, rhMIP-1δ, rhMIP-3 alpha, rhMIP-3 beta, and recombinant mouse (rm) MIP-1 alpha is observed. Neutralizes the biological activity of recombinant human CCL3/MIP‑1 alpha. In the chemotaxis assay this antibody will also partially neutralize the biological activity of rhMIP-1 beta at a 20 fold higher IgG concentration. It will not neutralize the biological activity of rmMIP-1 beta or rmMIP-1 alpha.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 2-8 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL3/MIP‑1 alpha isoform LD78a.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Clin. Chem., 2007;53(11):2010-2. Species: Human Sample Type: Plasma Application: ELISA Development Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. Authors: Pfleger C, Schloot N, ter Veld F J. Immunol. Methods, 2007;329(1):214-8. Species: Human Sample Type: Serum Application: Luminex Development HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P AIDS, 2007;21(10):1263-72. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma. Authors: Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio JO, Bergsagel PL, Stewart AK Blood, 2006;108(10):3465-71. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Gene copy number regulates the production of the human chemokine CCL3-L1. Authors: Townson JR, Barcellos LF, Nibbs RJ Eur. J. Immunol., 2002;32(10):3016-26. Species: Human Sample Type: Whole Cells Application: Neut Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes. Authors: Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R Virology, 2002;294(1):209-21. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human CCL3/MIP-1 alpha Ala27-Ala92 Accession # P10147
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8⁺ and CD4⁺ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

参考图片

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Human CCL3/
MIP‑1 alpha Antibody. Recombinant Human CCL3/MIP‑1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human
CCL3/MIP‑1 alpha (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP‑1 alpha Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF-270-NA). The ND₅₀ is typically 2-8 µg/mL.

CCL3/MIP‑1 alpha in Human Tonsil. CCL3/MIP‑1 alpha was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-270-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.