Human DPPIV/CD26 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human DPPIV/CD26 Affinity Purified Polyclonal Ab (100 UG)

货号： AF1180 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF1180
别名	ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5
全称	Dipeptidyl-peptidase IV
反应种属	Human
应用	Western Blot(0.2 µg/mL) Immunohistochemistry(1-15 µg/mL) Immunocytochemistry(1-15 µg/mL)
目标/特异性	Detects human DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse DPPIV is observed.
使用方法	Western Blot: 0.2 µg/mL Simple Western: 2 µg/mL Immunohistochemistry: 1-15 µg/mL Immunocytochemistry: 1-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1803 (Human); 13482 (Mouse); 102133935 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling. Authors: Lessard J, Pelletier M, Biertho L, Biron S, Marceau S, Hould F, Lebel S, Moustarah F, Lescelleur O, Marceau P, Tchernof A PLoS ONE, 2015;10(3):e0122065. Species: Human Sample Type: Cell Lysates Application: WB Generation of a transgenic mouse model of Middle East respiratory syndrome coronavirus infection and disease. Authors: Agrawal A, Garron T, Tao X, Peng B, Wakamiya M, Chan T, Couch R, Tseng C J Virol, 2015;89(7):3659-70. Species: Human Sample Type: Cell Lysates Application: WB Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes. Authors: Ohnuma K, Hatano R, Aune T, Otsuka H, Iwata S, Dang N, Yamada T, Morimoto C J Immunol, 2015;194(8):3697-712. Species: Mouse Sample Type: Whole Tissue Application: IHC - paraffin Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection. Authors: Peck K, Cockrell A, Yount B, Scobey T, Baric R, Heise M J Virol, 2015;89(8):4696-9. Species: Human Sample Type: Whole Cells Application: IHC Not Specified High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus. Authors: Scheuplein V, Seifried J, Malczyk A, Miller L, Hocker L, Vergara-Alert J, Dolnik O, Zielecki F, Becker B, Spreitzer I, Konig R, Becker S, Waibler Z, Muhlebach M J Virol, 2015;89(7):3859-69. Species: Human Sample Type: Whole Cells Application: Blocking Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus. Authors: Raj, V Stalin, Smits, Saskia L, Provacia, Lisette, van den Brand, Judith M, Wiersma, Lidewij, Ouwendijk, Werner J, Bestebroer, Theo M, Spronken, Monique, van Amerongen, Geert, Rottier, Peter J, Fouchier, Ron A M, Bosch, Berend J, Osterhaus, Albert D, Haagmans, Bart L J Virol, 2014;88(3):1834-8. Species: Ferret Sample Type: Whole Cells Application: Flow In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Authors: Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C Virol J, 2013;10(0):359. Species: Human Sample Type: Whole Tissue Application: IF CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion. Authors: Havre, Pamela A, Dang, Long H, Ohnuma, Kei, Iwata, Satoshi, Morimoto, Chikao, Dang, Nam H BMC Cancer, 2013;13(0):517. Species: Human Sample Type: Cell Lysates Application: WB Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function. Authors: Shin JW, Jurisic G, Detmar M Exp. Cell Res., 2008;314(16):3048-56. Species: Human Sample Type: Cell Lysates Application: WB Distinct gene expression-defined classes of gastrointestinal stromal tumor. Authors: Yamaguchi U, Nakayama R, Honda K, Ichikawa H, Hasegawa T, Shitashige M, Ono M, Shoji A, Sakuma T, Kuwabara H, Shimada Y, Sasako M, Shimoda T, Kawai A, Hirohashi S, Yamada T J. Clin. Oncol., 2008;26(25):4100-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26 (Catalog # 1180-SE) Asp34-Pro766 Accession # Q53TN1
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain. The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression. DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4⁺ cells. It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine. It degrades peptide hormones such as glucagon. It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Stem Cell Markers Co-stimulatory and Co-inhibitory Molecules Diabetes Human CD Marker Products ICC / IHC Images: DPPIV/CD26 Infectious Diseases Mouse Obesity Antibody Array Serine Proteases and Regulators

参考图片

Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DPPIV/CD26 in Human PBMCs. DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

DPPIV/CD26 in Human Psoriatic Skin. DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.