| 货号 | AF8537 |
| 别名 | A1B; A1BG; ABG; GAB |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1:1000 dilution) Simple Western(1:100 dilution) Immunocytochemistry(1:20-1:50 dilution) |
| 目标/特异性 | Detects mouse and rat alpha 1B-Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human alpha 1B-Glycoprotein observed. |
| 使用方法 | Western Blot: 1:1000 dilution Simple Western: 1:100 dilution Immunocytochemistry: 1:20-1:50 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1 (Human); 117586 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse alpha 1B-Glycoprotein Leu21-Gly512 Accession # Q19LI2 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 背景 | The alpha 1B-Glycoprotein is a plasma glycoprotein, encoded by the A1BG gene with an unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Mouse and Rat 1B-Glycoprotein at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
alpha 1B-Glycoprotein in Hepa 1‑6 Mouse Cell Line. alpha 1B-Glycoprotein was detected in immersion fixed Hepa 1‑6 mouse hepatoma cell line using Rabbit Anti-Mouse/Ratalpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) at 1:20 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Mouse and rat alpha 1B-Glycoprotein by Simple WesternTM. Simple Western lane view shows lysates mouse plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for alpha 1B-Glycoprotein at approximately 113 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Mouse/Rat alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
