| 货号 | AF8554 |
| 别名 | DKFZp781N0678; EC 1.1.1.205; IMP (inosine 5-monophosphate) dehydrogenase 1; IMP (inosine monophosphate) dehydrogenase 1; IMP dehydrogenase 1; IMPD 1; IMPD; IMPD1; IMPDH 1; IMPDH1; IMPDH-I; inosine-5-monophosphate dehydrogenase 1; LCA11; LCA11RP10; retinitis pigmentosa 10 (autosomal dominant); RP10; sWSS2608 | 全称 | Inosine 5-Monophosphate Dehydrogenase 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1:1000 dilution) Immunocytochemistry(1:200 dilution) |
| 目标/特异性 | Detects human, rat, and mouse IMP Dehydrogenase 1/IMPDH1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IMPDH2 is observed. |
| 使用方法 | Western Blot: 1:1000 dilution Immunocytochemistry: 1:200 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3614 (Human); 23917 (Mouse); 362329 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf21 derived recombinaint human IMP Dehydrogenase 1/IMPDH1 Met1-Tyr563 (predicted) Accession # P20839 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 背景 | The enzyme IMPDH1 (Inosine-5-monophosphate dehydrogenase 1) catalyzes the conversion of inosine 5-phosphate (IMP) to xanthosine 5-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.The enzyme is 514 aminoacids long, with a predicted molecular weight of 55 kDa. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human and Mouse IMP Dehydrogenase 1/IMPDH1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for IMP Dehydrogenase 1/IMPDH1 at approximately 55-57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
IMP Dehydrogenase 1/IMPDH1 in HeLa Human Cervical Epithelial Carcinoma Cell Line. IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) at a 1:200 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
